Literature DB >> 7868646

Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus.

V M Carn1, R P Kitching, J M Hammond, P Chand.   

Abstract

The gene coding for the capripoxvirus structural protein P32 was cloned, expressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and purified on glutathione Sepharose. An indirect enzyme linked immunosorbent assay (ELISA) using this antigen was developed to screen bovine sera for antibodies to capripoxvirus. Sequential serum samples from experimentally infected animals tested by ELISA and by virus neutralisation test (VNT) showed that the ELISA was more sensitive and detected antibodies to capripoxvirus earlier post-infection than the VNT.

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Year:  1994        PMID： 7868646     DOI： 10.1016/0166-0934(94)90143-0

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

1.  Phylogenetic analysis of the lumpy skin disease viruses in northwest of Iran.

Authors:  Payman Sameea Yousefi; Bahram Dalir-Naghadeh; Karim Mardani; Ghader Jalilzadeh-Amin
Journal:  Trop Anim Health Prod       Date:  2018-06-13       Impact factor: 1.559

2.  Sensitive and Specific Detection of Lumpy Skin Disease Virus in Cattle by CRISPR-Cas12a Fluorescent Assay Coupled with Recombinase Polymerase Amplification.

Authors:  Chuanwen Jiang; Dagang Tao; Yuanchen Geng; Hao Yang; Bingrong Xu; Yingyu Chen; Changmin Hu; Huanchun Chen; Shengsong Xie; Aizhen Guo
Journal:  Genes (Basel)       Date:  2022-04-22       Impact factor: 4.141

3.  Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32.

Authors:  Hong Tian; Yan Chen; Jinyan Wu; Youjun Shang; Xiangtao Liu
Journal:  Virol J       Date:  2010-09-21       Impact factor: 4.099

4.  Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA.

Authors:  Milovan Milovanović; Vesna Milićević; Sonja Radojičić; Miroslav Valčić; Bernd Hoffmann; Klaas Dietze
Journal:  Virol J       Date:  2020-03-05       Impact factor: 4.099

5.  Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene.

Authors:  Madhusudan Hosamani; Bimalendu Mondal; Prabhakar A Tembhurne; Santanu Kumar Bandyopadhyay; Raj Kumar Singh; Thaha Jamal Rasool
Journal:  Virus Genes       Date:  2004-08       Impact factor: 2.198

6.  Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies.

Authors:  Olga V Chervyakova; Valentin L Zaitsev; Bulat K Iskakov; Elmira T Tailakova; Vitaliy M Strochkov; Kulyaisan T Sultankulova; Nurlan T Sandybayev; Gulshan E Stanbekova; Daniyar K Beisenov; Yergali O Abduraimov; Muratbay Mambetaliyev; Abylay R Sansyzbay; Natalia Y Kovalskaya; Lev G Nemchinov; Rosemarie W Hammond
Journal:  Viruses       Date:  2016-06-07       Impact factor: 5.048

Review 7.  Review: Capripoxvirus Diseases: Current Status and Opportunities for Control.

Authors:  E S M Tuppurainen; E H Venter; J L Shisler; G Gari; G A Mekonnen; N Juleff; N A Lyons; K De Clercq; C Upton; T R Bowden; S Babiuk; L A Babiuk
Journal:  Transbound Emerg Dis       Date:  2015-11-13       Impact factor: 5.005
  7 in total

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