Literature DB >> 7867070

Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

J O Lee1, P Rieu, M A Arnaout, R Liddington.   

Abstract

We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule. We suggest that this site represents a general metal ion-dependent adhesion site (MIDAS) for binding protein ligands. We further propose that the beta subunits of integrins contain a MIDAS motif within a modified A domain. Our crystal structure will allow reliable models to be built for other members of the A domain superfamily and should facilitate development of novel adhesion modulatory drugs.

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Year:  1995        PMID： 7867070     DOI： 10.1016/0092-8674(95)90517-0

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  217 in total

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Authors:  S S Rebello; J Huang; J D Faul; B R Lucchesi
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3.  Structure of adenovirus complexed with its internalization receptor, alphavbeta5 integrin.

Authors:  C Y Chiu; P Mathias; G R Nemerow; P L Stewart
Journal:  J Virol       Date:  1999-08       Impact factor: 5.103

4.  Locking in alternate conformations of the integrin alphaLbeta2 I domain with disulfide bonds reveals functional relationships among integrin domains.

Authors:  C Lu; M Shimaoka; Q Zang; J Takagi; T A Springer
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-27       Impact factor: 11.205

5.  Crystal structure of the extracellular segment of integrin alpha Vbeta3.

Authors:  J P Xiong; T Stehle; B Diefenbach; R Zhang; R Dunker; D L Scott; A Joachimiak; S L Goodman; M A Arnaout
Journal:  Science       Date:  2001-09-06       Impact factor: 47.728

6.  Engineered allosteric mutants of the integrin alphaMbeta2 I domain: structural and functional studies.

Authors:  Clare J McCleverty; Robert C Liddington
Journal:  Biochem J       Date:  2003-05-15       Impact factor: 3.857

Review 7.  Distribution and evolution of von Willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere.

Authors:  Charles A Whittaker; Richard O Hynes
Journal:  Mol Biol Cell       Date:  2002-10       Impact factor: 4.138

8.  Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds.

Authors:  Motomu Shimaoka; Chafen Lu; Azucena Salas; Tsan Xiao; Junichi Takagi; Timothy A Springer
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-04       Impact factor: 11.205

9.  Reversibly locking a protein fold in an active conformation with a disulfide bond: integrin alphaL I domains with high affinity and antagonist activity in vivo.

Authors:  M Shimaoka; C Lu; R T Palframan; U H von Andrian; A McCormack; J Takagi; T A Springer
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

10.  A statin-based inhibitor of lymphocyte function antigen-1 protects against ischemia/reperfusion-induced leukocyte adhesion in the colon.

Authors:  Min Xiu Wan; Rene Schramm; Daniel Klintman; Karl Welzenbach; Gabriele Weitz-Schmidt; Henrik Thorlacius
Journal:  Br J Pharmacol       Date:  2003-08-26       Impact factor: 8.739
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