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Literature DB >> 7866872

Genetic transformation of plants by protoplast electroporation.

Abstract

This article describes an optimized protocol for the electroporation of tobacco mesophyll protoplasts together with notes and data on the effects of various parameters and suggestions for work with protoplasts of other species. In this protocol, electroporation is achieved by means of electrical pulses from a high-voltage, capacitive-discharge unit. Procedures are described for measurement of protoplast viability with Evan's blue, the detection of transient expression of CAT and GUS gene plasmid constructs, and for the recovery of stable transformants based on selection for kanamycin resistance.

Entities: Chemical Disease Species

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Year: 1994 PMID： 7866872 DOI： 10.1007/bf02824806

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

13 in total

1. Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

Authors: K Lindsey; M G Jones
Journal: Plant Mol Biol Date: 1987-01 Impact factor: 4.076

2. Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization.

Authors: G W Bates; S A Carle; W C Piastuch
Journal: Plant Mol Biol Date: 1990-06 Impact factor: 4.076

3. Stable transformation of maize after gene transfer by electroporation.

Authors: M E Fromm; L P Taylor; V Walbot
Journal: Nature Date: 1986 Feb 27-Mar 5 Impact factor: 49.962

4. Electropore diameters, lifetimes, numbers, and locations in individual erythrocyte ghosts.

Authors: A E Sowers; M R Lieber
Journal: FEBS Lett Date: 1986-09-15 Impact factor: 4.124

5. Genetically transformed maize plants from protoplasts.

Authors: C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal: Science Date: 1988-04-08 Impact factor: 47.728

Review 6. Electric field-mediated fusion and related electrical phenomena.

Authors: U Zimmermann
Journal: Biochim Biophys Acta Date: 1982-11-30

7. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

Authors: R D Shillito; J Paszkowski; I Potrykus
Journal: Plant Cell Rep Date: 1983-10 Impact factor: 4.570

8. Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors: H Potter; L Weir; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1984-11 Impact factor: 11.205

Genetic transformation of plants by protoplast electroporation.

1. Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

2. Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization.

3. Stable transformation of maize after gene transfer by electroporation.

4. Electropore diameters, lifetimes, numbers, and locations in individual erythrocyte ghosts.

5. Genetically transformed maize plants from protoplasts.

Review 6. Electric field-mediated fusion and related electrical phenomena.

7. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

8. Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

9. Introduction of deoxyribonucleoside triphosphates into intact cells by electroporation.

10. Factors affecting protoplast electrofusion efficiency.

Review 1. Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.

2. Suitable transfection methods for single particle tracing in plant suspension cells.