| Literature DB >> 7866870 |
T Kapros1, A J Robertson, J H Waterborg.
Abstract
A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments.Mesh：
Substances：
Year: 1994 PMID： 7866870 DOI： 10.1007/bf02789291
Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695