Literature DB >> 7866866

Reporter enzymes for the study of promoter activity.

K Pardy.   

Abstract

This article describes the use of three reporter enzymes used to study promoter activity in transgenic animals. Chloramphenicol acetyl transferase may be assayed by a nonchromatographic method that is rapid and sensitive. beta-Galactosidase is measured by a photometric assay and luciferase is assayed by measuring the emission of light using a luminometer. The relative merits of each enzyme is discussed. The use of reporter enzymes provides a rapid and sensitive method for analysis of transgene expression.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7866866     DOI： 10.1007/BF02789287

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  7 in total

1.  In situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ gene.

Authors:  D R Goring; J Rossant; S Clapoff; M L Breitman; L C Tsui
Journal:  Science       Date:  1987-01-23       Impact factor: 47.728

2.  Grafting genetically modified cells into the rat brain: characteristics of E. coli beta-galactosidase as a reporter gene.

Authors:  S Shimohama; M B Rosenberg; A M Fagan; J A Wolff; M P Short; X O Breakefield; T Friedmann; F H Gage
Journal:  Brain Res Mol Brain Res       Date:  1989-06

3.  Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines.

Authors:  A R Brasier; J E Tate; J F Habener
Journal:  Biotechniques       Date:  1989 Nov-Dec       Impact factor: 1.993

4.  A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.

Authors:  M J Sleigh
Journal:  Anal Biochem       Date:  1986-07       Impact factor: 3.365

5.  Firefly luciferase luminescence assays using scintillation counters for quantitation in transfected mammalian cells.

Authors:  V T Nguyen; M Morange; O Bensaude
Journal:  Anal Biochem       Date:  1988-06       Impact factor: 3.365

6.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors:  C M Gorman; L F Moffat; B H Howard
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

7.  Firefly luciferase gene: structure and expression in mammalian cells.

Authors:  J R de Wet; K V Wood; M DeLuca; D R Helinski; S Subramani
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272
  7 in total
  2 in total

1.  Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.

Authors:  A J Clark; G Harold; F E Yull
Journal:  Nucleic Acids Res       Date:  1997-03-01       Impact factor: 16.971

2.  Identification of the Talaromyces cellulolyticus Gene Encoding an Extracellular Enzyme with β-galactosidase Activity and Testing it as a Reporter for Gene Expression Assays.

Authors:  Alexandra P Orleneva; Vsevolod A Serebrianyi; Ekaterina A Kutukova; Tatiana A Yampolskaya
Journal:  Mol Biotechnol       Date:  2022-01-20       Impact factor: 2.695
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.