The expulsion of Echinostoma trivolvis (Trematoda) from ICR mice: extension/retraction mechanisms and ultrastructure of the collar spines.

Ultrastructural observations of collar spines and surrounding tissues associated with extension/retraction mechanisms of the spines were made on Echinostoma trivolvis recovered from ICR mice or golden hamsters. The spine consisted mainly of a homogeneous, crystalline structure of moderate to heavy electron density. The spine was surrounded by an outer layer of tegument-like material. Interstitial material was located between this outer layer and the surrounding tegument. Muscle bundles associated with spine movements were anchored by hemidesmosomes to interstitial material. Contraction of these muscles caused an invagination of the tegument surrounding a spine, resulting in spinal protrusion. Relaxation of these muscles resulted in spinal retraction. An immunocytochemistry technique using colloidal gold confirmed the presence of actin in the collar spines. Most of the collar spines of excysted metacercariae and juveniles maintained in a defined medium supplemented with fresh hamster serum were extended, whereas worms maintained in mouse serum mainly showed retracted spines. Apparently, factors in mouse serum are involved in spinal retraction.