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Literature DB >> 785471

In vitro system for induction of delayed early RNA of bacteriophage T4.

C Thermes, P Daegelen, V De Franciscis, E Brody.

Abstract

Concentrated lysates of Escherichia coli that had been infected with bacteriophage T4 in the presence of chloramphenicol show the same restriction of transcription in vitro as is found in vivo. Restricted lysates can be complemented with lysates from infected cells to induce production of delayed early RNA. Complementation takes place between the RNA polymerase of the restricted lysate and the DNA of the unrestricted lysate. We present evidence that delayed early RNA in these lysates is initiated at quasi-late (middle) promoters, and that such recognition is related to changes in the state of the template DNA.

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Year: 1976 PMID： 785471 PMCID： PMC430689 DOI： 10.1073/pnas.73.8.2569

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

In vitro system for induction of delayed early RNA of bacteriophage T4.

1. Transcription and translation of prereplicative bacteriophage T4 genes in vitro.

2. Bacteriophage T4 gene expression. Evidence for two classes of prereplicative cistrons.

3. Transcription in vitro using mixtures of ethylene glycol and water.

4. Mechanism of ethidium bromide inhibition of RNA polymerase.

5. Bacteriophage sigma factor for RNA polymerase.

6. Two modes of in vivo transcription for genes 43 and 45 of phage T4.

7. Transcription of the bacteriophage T4 template. Detailed comparison of in vitro and in vivo transcripts.

8. Transcription of the bacteriophage T4 template. Obligate synthesis of T4 prereplicative RNA in vitro.

9. A new method for selecting RNA polymerase mutants.

10. Regulation of rII and region D transcription in T4 bacteriophage: a sucrose gradient analysis.

Review 1. Role of ribonucleic acid polymerase in gene selection in procaryotes.

2. In vivo expression of the rII region of bacteriophage T4 present in chimeric plasmids.

3. Changes in the promoter range of RNA polymerase resulting from bacteriophage T4-induced modification of core enzyme.

4. Bacteriophage T4 gol site: sequence analysis and effects of the site on plasmid transformation.

5. Genetic complementation by cloned bacteriophage T4 late genes.

6. Identification and biosynthesis of the bacteriophage T4 mot regulatory protein.