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Literature DB >> 7852589

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results.

Abstract

The Pathfinder respiratory syncytial virus (RSV) enzyme immunoassay (EIA) (Kallestad), the shell vial (SV) technique, and conventional cell culture (CC) were compared for detection of RSV in nasopharyngeal aspirates. We found sensitivities, specificities, and positive and negative predictive values of 58.4, 100, 100, and 68.2%; 80.7, 97.2, 97.0, and 81.9%; and 77.6, 97.2, 96.9, and 79.5% for the CC, EIA, and SV methods, respectively. The SV and EIA techniques were both more sensitive than CC (P < 0.001). Finally, 29 respiratory viruses other than RSV were identified by CC.

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Culture Media

Year: 1994 PMID： 7852589 PMCID： PMC264177 DOI： 10.1128/jcm.32.11.2861-2864.1994

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Enhanced isolation of respiratory syncytial virus in cell culture.

Authors: M Q Arens; E M Swierkosz; R R Schmidt; T Armstrong; K A Rivetna
Journal: J Clin Microbiol Date: 1986-04 Impact factor: 5.948

2. Comparison of two enzyme-linked immunosorbent assay (EIA) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (RSV).

Authors: E K Subbarao; N J Whitehurst; J L Waner
Journal: Diagn Microbiol Infect Dis Date: 1987-12 Impact factor: 2.803

3. Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool.

Authors: G P Rabalais; G G Stout; K L Ladd; K M Cost
Journal: J Clin Microbiol Date: 1992-06 Impact factor: 5.948

4. Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens.

Authors: E M Swierkosz; R Flanders; L Melvin; J D Miller; M W Kline
Journal: J Clin Microbiol Date: 1989-06 Impact factor: 5.948

5. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay.

Authors: B A Lauer; H A Masters; C G Wren; M J Levin
Journal: J Clin Microbiol Date: 1985-11 Impact factor: 5.948

6. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.

Authors: K Bromberg; G Tannis; B Daidone; L Clarke; M Sierra
Journal: J Clin Microbiol Date: 1987-02 Impact factor: 5.948

7. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.

Authors: P D Swenson; M H Kaplan
Journal: J Clin Microbiol Date: 1986-03 Impact factor: 5.948

8. Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.

Authors: J H Hughes; D R Mann; V V Hamparian
Journal: J Clin Microbiol Date: 1988-03 Impact factor: 5.948

9. Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens.

Authors: G S Ahluwalia; G W Hammond
Journal: Diagn Microbiol Infect Dis Date: 1988-03 Impact factor: 2.803

10. Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay.

Authors: A Hornsleth; E Brenøe; B Friis; F U Knudsen; P Uldall
Journal: J Clin Microbiol Date: 1981-11 Impact factor: 5.948

7 in total

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results.

1. Enhanced isolation of respiratory syncytial virus in cell culture.

2. Comparison of two enzyme-linked immunosorbent assay (EIA) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (RSV).

3. Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool.

4. Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens.

5. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay.

6. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.

7. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.

8. Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.

9. Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens.

10. Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay.

1. Detection of respiratory syncytial virus in samples frozen at -20 degrees C.

2. Microwave-accelerated direct immunofluorescent staining for respiratory syncytial virus and influenza A virus.

Review 3. Respiratory Syncytial Virus: Infection, Detection, and New Options for Prevention and Treatment.

Review 4. Ag nanorod based surface-enhanced Raman spectroscopy applied to bioanalytical sensing.

5. Diagnosis of respiratory syncytial virus infection.

6. Defining the timing of respiratory syncytial virus (RSV) outbreaks: an epidemiological study.

Review 7. Developments in tissue culture detection of respiratory viruses.