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Literature DB >> 785233

Conformational peculiarities of tRNAMetf from E. coli as revealed by fluorescent methods.

Abstract

Conformational transitions in several individual tRNAs (tRNAMetf, tRNAPhe from E. coli, tRNAVal1, tRNASer, tRNAPhe from yeast) have been studied under various environmental conditions. The binding isotherms studies for dyes-tRNA complexes exhibited similarities in conformational states of all tRNAs investigated at low ionic strength (0.01 M NaCl). By contrast, at high ionic strength (0.4 M NaCl or 2 X 10(-4) M Mg2+) a marked difference is found in structural features of tRNAMetf as compared with other tRNAs used. The tRNAMetf is the only tRNA species that does not reveal the strong type of complexes with ethidium bromide, acriflavine and acridine orange.

Entities: Chemical Species

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Year: 1976 PMID： 785233 DOI： 10.1007/bf00356938

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

14 in total

1. Identification of a unique ethidium bromide binding site on yeast tRNAPhe by high resolution (300 MHz) nuclear magnetic resonance.

Authors: C R Jones; D R Kearns
Journal: Biochemistry Date: 1975-06-17 Impact factor: 3.162

2. Cooperative binding of magnesium to transfer ribonucleic acid studied by a fluorescent probe.

Authors: D C Lynch; P R Schimmel
Journal: Biochemistry Date: 1974-04-23 Impact factor: 3.162

3. The binding of ethidium bromide to different conformations of tRNA. Unfolding of tertiary structure.

Authors: C Urbanke; R Römer; G Maass
Journal: Eur J Biochem Date: 1973-03-15

4. The involvement of 5S RNA in the binding of tRNA to ribosomes.

Authors: V A Erdmann; M Sprinzl; O Pongs
Journal: Biochem Biophys Res Commun Date: 1973-10-01 Impact factor: 3.575

5. Interaction between proflavine and double stranded polynucleotides.

Authors: V Deubel; M Leng
Journal: Biochimie Date: 1974 Impact factor: 4.079

6. Studies of the binding of ethidium bromide to transfer ribonucleic acid: absorption, fluorescence, ultracentrifugation and kinetic investigations.

Authors: R Bittman
Journal: J Mol Biol Date: 1969-12-14 Impact factor: 5.469

Conformational peculiarities of tRNAMetf from E. coli as revealed by fluorescent methods.

1. Identification of a unique ethidium bromide binding site on yeast tRNAPhe by high resolution (300 MHz) nuclear magnetic resonance.

2. Cooperative binding of magnesium to transfer ribonucleic acid studied by a fluorescent probe.

3. The binding of ethidium bromide to different conformations of tRNA. Unfolding of tertiary structure.

4. The involvement of 5S RNA in the binding of tRNA to ribosomes.

5. Interaction between proflavine and double stranded polynucleotides.

6. Studies of the binding of ethidium bromide to transfer ribonucleic acid: absorption, fluorescence, ultracentrifugation and kinetic investigations.

7. The binding of proflavine to transfer ribonucleic acid: dependence on secondary structure.

8. Circular dichroism studies of deoxyribonucleic acid complexes with arginine-rich histone IV (f2al).

9. Structural requirements for the binding of ethidium to nucleic acids.

10. Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain.

1. Structural investigation of nuclear RNP particles containing pre-mRNA by different fluorescence techniques.