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Literature DB >> 785221

Insertion sequence IS2 near the gene for prophage lambda excision.

E Mosharrafa, W Pilacinski, J Zissler, M Fiandt, W Szybalski.

Abstract

In this study we characterize a variant of the lambdacI857S7 prophage, designated lambdabi2cI857S7, which carries a DNA insertion. The insertion sequence is IS2, and it resides in the antipolar orientation II just upstream from the gene for prophage excision (xis) at 61.6%lambda. This bi2 insertion mutant could prove valuable for studies on possible recombination functions of IS2 DNA and of its effect on the lambda integration and excision functions.

Entities: Species

Mesh：

Substances：
DNA, Viral

Year: 1976 PMID： 785221 DOI： 10.1007/bf00337943

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

36 in total

1. Physical mapping of coliphage lambda att2.

Authors: M Fiandt; M E Gottesman; M J Shulman; E H Szybalski; W Szybalski; R A Weisberg
Journal: Virology Date: 1976-07-01 Impact factor: 3.616

2. Electron microscopy of polar insertions in the lac operon of Escherichia coli.

Authors: M H Malamy; M Fiandt; W Szybalski
Journal: Mol Gen Genet Date: 1972

3. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

Authors: M Fiandt; W Szybalski; M H Malamy
Journal: Mol Gen Genet Date: 1972

4. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors: R B Helling; H M Goodman; H W Boyer
Journal: J Virol Date: 1974-11 Impact factor: 5.103

1. Deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.

Authors: P Nisen; M Purucker; L Shapiro
Journal: J Bacteriol Date: 1979-11 Impact factor: 3.490

Insertion sequence IS2 near the gene for prophage lambda excision.

1. Physical mapping of coliphage lambda att2.

2. Electron microscopy of polar insertions in the lac operon of Escherichia coli.

3. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

4. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

5. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.

6. Mutations of coliphage lambda affecting the expression of replicative functions O and P.

7. O0 and strong-polar mutations in the gal operon are insertions.

8. Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations.

9. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.

10. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.

1. Deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.

2. Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences.

3. DNA tertiary structures formed in vitro by misaligned hybridization of multiple tandem repeat sequences.

4. Transposition of IS2 into the hemB gene of Escherichia coli K-12.

5. Transcription initiation sites within an IS2 insertion in a Gal-constitutive mutant of Escherichia coli.