Literature DB >> 7851967

Evaluation of PCR based test for the detection of Mycobacterium tuberculosis in coded sputum specimens.

P Khandekar1, A G Amin, P P Reddi, J N Banwalikar, T Shivannaure, V M Katoch.   

Abstract

A test based on amplification of 169 bp DNA specific to Mycobacterium tuberculosis was evaluated in a trial, on 50 coded samples which included 25 sputum specimens from radiologically, clinically and bacteriologically proven patients of pulmonary tuberculosis and 25 control specimens. At the end of the trial the code was broken and the results of PCR test were compared with those obtained with Ziehl Neelsen staining and culture test. The test appeared highly sensitive reacting 100 per cent in either of the hands with concordance rate of 76 per cent; 3 of 25 control samples gave false positive results.

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Year:  1994        PMID: 7851967

Source DB:  PubMed          Journal:  Indian J Med Res        ISSN: 0971-5916            Impact factor:   2.375


  6 in total

1.  PCR-based rapid detection of Mycobacterium tuberculosis in blood from immunocompetent patients with pulmonary tuberculosis.

Authors:  N Ahmed; A K Mohanty; U Mukhopadhyay; V K Batish; S Grover
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

2.  DNA AMPLIFICATION OF A REPETITIVE SEQUENCE - IS 6110 IN THE EARLY DIAGNOSIS OF EXTRA PULMONARY TUBERCULOSIS.

Authors:  K K Lahiri; Y K Goorha
Journal:  Med J Armed Forces India       Date:  2011-07-21

3.  Development of a two-step nucleic acid amplification test for accurate diagnosis of the Mycobacterium tuberculosis complex.

Authors:  Chien-Ru Lin; Hsin-Yao Wang; Ting-Wei Lin; Jang-Jih Lu; Jason Chia-Hsun Hsieh; Min-Hsien Wu
Journal:  Sci Rep       Date:  2021-03-11       Impact factor: 4.379

4.  Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples.

Authors:  Thongchai Kaewphinit; Narong Arunrut; Wansika Kiatpathomchai; Somchai Santiwatanakul; Pornpun Jaratsing; Kosum Chansiri
Journal:  Biomed Res Int       Date:  2013-03-05       Impact factor: 3.411

5.  Rapid diagnosis of Mycobacterium tuberculosis with Truenat MTB: a near-care approach.

Authors:  Chaitali Nikam; Manjula Jagannath; Manoj Mulakkapurath Narayanan; Vinaya Ramanabhiraman; Mubin Kazi; Anjali Shetty; Camilla Rodrigues
Journal:  PLoS One       Date:  2013-01-21       Impact factor: 3.240

6.  Ultrasensitive detection of Mycobacterium tuberculosis by a rapid and specific probe-triggered one-step, simultaneous DNA hybridization and isothermal amplification combined with a lateral flow dipstick.

Authors:  Wansadaj Jaroenram; Jantana Kampeera; Narong Arunrut; Sarawut Sirithammajak; Sarinya Jaitrong; Kobporn Boonnak; Pakapreud Khumwan; Therdsak Prammananan; Angkana Chaiprasert; Wansika Kiatpathomchai
Journal:  Sci Rep       Date:  2020-10-12       Impact factor: 4.379

  6 in total

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