Mutations which reduce levels of pyruvate dehydrogenase in Schizosaccharomyces pombe cause a requirement for arginine or glutamine.

Forty-four mutants of Schizosaccharomyces pombe were isolated which required supplementation with arginine or glutamine. These mutants appear to define three genes, provisionally named agg1, agg2 and agg3 (arginine, glutamine requiring). Mutants in all three genes were found to have reduced levels of pyruvate dehydrogenase compared to wild-type.