BACKGROUND: A unique case of primary testicular lymphoma in a child is reported. METHODS: Tumor tissue was studied using immunohistochemical techniques and southern blot hybridization to detect immunoglobulin and bcl-2 gene rearrangement and in situ hybridization for the Epstein-Barr virus (EBV) genome. RESULTS: Light microscopy revealed a lymphocytic infiltrate with a follicular pattern. Immunohistochemical staining revealed lambda light chain restriction and gene rearrangement studies revealed a clonal rearrangement of the immunoglobulin heavy chain, confirming a clonal neoplastic process. Immunostaining failed to detect bcl-2 protein expression, and no evidence of bcl-2 gene rearrangement was noted on southern blot analysis. In situ hybridization for EBV nucleic acid in tumor tissue was negative. CONCLUSIONS: To the authors' knowledge, this is the first report of a case of a primary testicular lymphoma with follicular histology in a child. Despite the follicular histology, no evidence of bcl-2 expression or gene rearrangement was detected.
BACKGROUND: A unique case of primary testicular lymphoma in a child is reported. METHODS:Tumor tissue was studied using immunohistochemical techniques and southern blot hybridization to detect immunoglobulin and bcl-2 gene rearrangement and in situ hybridization for the Epstein-Barr virus (EBV) genome. RESULTS: Light microscopy revealed a lymphocytic infiltrate with a follicular pattern. Immunohistochemical staining revealed lambda light chain restriction and gene rearrangement studies revealed a clonal rearrangement of the immunoglobulin heavy chain, confirming a clonal neoplastic process. Immunostaining failed to detect bcl-2 protein expression, and no evidence of bcl-2 gene rearrangement was noted on southern blot analysis. In situ hybridization for EBV nucleic acid in tumor tissue was negative. CONCLUSIONS: To the authors' knowledge, this is the first report of a case of a primary testicular lymphoma with follicular histology in a child. Despite the follicular histology, no evidence of bcl-2 expression or gene rearrangement was detected.
Authors: Mark A Lones; Martine Raphael; Keith McCarthy; Andrew Wotherspoon; Marie-Josee Terrier-Lacombe; Alan D Ramsay; Ken Maclennan; Mitchell S Cairo; Mary Gerrard; Jean Michon; Catherine Patte; Ross Pinkerton; Leonard Sender; Anne Auperin; Richard Sposto; Claire Weston; Nyla A Heerema; Warren G Sanger; Daniel von Allmen; Sherrie L Perkins Journal: J Pediatr Hematol Oncol Date: 2012-01 Impact factor: 1.289
Authors: Leticia Quintanilla-Martinez; Birgitta Sander; John K C Chan; Luc Xerri; German Ott; Elias Campo; Steven H Swerdlow Journal: Virchows Arch Date: 2015-09-28 Impact factor: 4.064
Authors: S A Pileri; E Sabattini; P Rosito; P L Zinzani; S Ascani; G Fraternali-Orcioni; B Gamberi; M Piccioli; D Vivenza; B Falini; G Gaidano Journal: J Clin Pathol Date: 2002-09 Impact factor: 3.411