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Literature DB >> 7849399

Use of porcine interspersed repeat sequences in PCR-mediated genotyping.

Abstract

PCR primers derived from porcine short and long interspersed repeat sequences were used to amplify DNA samples isolated from individual members of three-generation pig reference pedigrees. Subsequent high-resolution gel electrophoresis of both SINE and LINE-PCR products allowed direct visualisation of polymorphisms that segregated in a Mendelian manner. Additional polymorphisms were detected by Southern blotting of the gels described above followed by hybridization with simple sequence DNA. Genotyping by interspersed repeat-PCR exploits the natural architecture of the pig genome and allows the typing of polymorphisms by utilizing pre-existing sequence information.

Entities: Species

Mesh：

Substances：
DNA Primers

Year: 1994 PMID： 7849399 DOI： 10.1007/bf00411458

Source DB: PubMed Journal: Mamm Genome ISSN： 0938-8990 Impact factor: 2.957

21 in total

1. 3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

Authors: J P Charlieu; A M Laurent; D A Carter; M Bellis; G Roizès
Journal: Nucleic Acids Res Date: 1992-03-25 Impact factor: 16.971

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross.

Authors: R D Cox; N G Copeland; N A Jenkins; H Lehrach
Journal: Genomics Date: 1991-06 Impact factor: 5.736

4. Alumorphs--human DNA polymorphisms detected by polymerase chain reaction using Alu-specific primers.

Authors: D Sinnett; J M Deragon; L R Simard; D Labuda
Journal: Genomics Date: 1990-07 Impact factor: 5.736

5. Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.

Authors: A L Boyle; S G Ballard; D C Ward
Journal: Proc Natl Acad Sci U S A Date: 1990-10 Impact factor: 11.205

2. Pig genome analysis: differential distribution of SINE and LINE sequences is less pronounced than in the human and mouse genomes.

Authors: P D Thomsen; J R Miller
Journal: Mamm Genome Date: 1996-01 Impact factor: 2.957

3. [Biomechanics of cartilage tissue engineering constructs : Sensitive test procedure for assessment of biomechanical functionality and further development after in vivo transplantation].

Authors: A Krase; E Steck; W Roth; W Richter
Journal: Orthopade Date: 2013-04 Impact factor: 1.087

3 in total

Use of porcine interspersed repeat sequences in PCR-mediated genotyping.

1. 3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. Interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross.

4. Alumorphs--human DNA polymorphisms detected by polymerase chain reaction using Alu-specific primers.

5. Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.

6. Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

7. Identification and DNA sequence of an interspersed repetitive DNA element in the genome of the miniature swine.

8. The development of sequence-tagged sites for human chromosome 4.

9. 5' and 3' SINE-PCR allows genotyping of pig families without cloning and sequencing steps.

10. Short interspersed nuclear element (SINE) sequences of the Bovidae.

1. Mapping dispersed repetitive loci using semi-specific PCR cloning and somatic cell hybrid mapping.

2. Pig genome analysis: differential distribution of SINE and LINE sequences is less pronounced than in the human and mouse genomes.

3. [Biomechanics of cartilage tissue engineering constructs : Sensitive test procedure for assessment of biomechanical functionality and further development after in vivo transplantation].