OBJECTIVE: To determine if the size of human oocytes at collection from unstimulated ovaries is related to their ability to resume meiosis and undergo maturation in vitro. DESIGN: A comparative study of oocyte diameter at collection. SETTING: Department of Obstetrics and Gynecology, Northwestern University Medical School. PATIENTS: Women age 25 to 39 years of age undergoing gynecological procedures yielding oophorectomy specimens. INTERVENTION: Oocytes obtained from ovarian tissue were cultured in Ham's F-10 and fetal bovine serum for 72 hours and observed two times per day. MAIN OUTCOME MEASURE: The oocytes ability to resume meiosis and complete maturation based on their diameter at collection. RESULTS: Chi-squared analysis revealed a significant difference in oocytes measuring 86 to 105 microns versus those measuring 106 to 125 microns. CONCLUSION: The unstimulated human oocyte appears to have a size-dependent ability to resume meiosis and complete maturation.
OBJECTIVE: To determine if the size of human oocytes at collection from unstimulated ovaries is related to their ability to resume meiosis and undergo maturation in vitro. DESIGN: A comparative study of oocyte diameter at collection. SETTING: Department of Obstetrics and Gynecology, Northwestern University Medical School. PATIENTS: Women age 25 to 39 years of age undergoing gynecological procedures yielding oophorectomy specimens. INTERVENTION: Oocytes obtained from ovarian tissue were cultured in Ham's F-10 and fetal bovine serum for 72 hours and observed two times per day. MAIN OUTCOME MEASURE: The oocytes ability to resume meiosis and complete maturation based on their diameter at collection. RESULTS: Chi-squared analysis revealed a significant difference in oocytes measuring 86 to 105 microns versus those measuring 106 to 125 microns. CONCLUSION: The unstimulated human oocyte appears to have a size-dependent ability to resume meiosis and complete maturation.
Authors: Kerri L Marquard; Sahar M Stephens; Emily S Jungheim; Valerie S Ratts; Randall R Odem; Susan Lanzendorf; Kelle H Moley Journal: Fertil Steril Date: 2010-11-11 Impact factor: 7.329
Authors: Francesca E Duncan; Karen Schindler; Richard M Schultz; Cecilia S Blengini; Paula Stein; Stephen A Stricker; Gary M Wessel; Carmen J Williams Journal: Mol Hum Reprod Date: 2020-11-01 Impact factor: 4.518