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Literature DB >> 7841794

Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver.

Abstract

CMP-N-acetylneuraminate hydroxylase was isolated from mouse liver high speed supernatant with a yield of 0.4% and an apparent 1000-fold purification. The enzyme is a monomeric protein with a molecular weight of 66 kDa, as determined by gel filtration and SDS-PAGE. The hydroxylase system was reconstituted with Triton X-100-solubilized mouse liver microsomes and purified soluble or microsomal forms of cytochrome b5 reductase and cytochrome b5. The systems were characterized in detail and kinetic parameters for each system were determined.

Entities: Chemical Gene Species

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Year: 1994 PMID： 7841794 DOI： 10.1007/bf00731218

Source DB: PubMed Journal: Glycoconj J ISSN： 0282-0080 Impact factor: 2.916

26 in total

1. Spectrum of horse-heart cytochrome c.

Authors: E MARGOLIASH; N FROHWIRT
Journal: Biochem J Date: 1959-03 Impact factor: 3.857

2. Peroxides in detergents as interfering factors in biochemical analysis.

Authors: M Lever
Journal: Anal Biochem Date: 1977-11 Impact factor: 3.365

3. Mouse liver cytidine-5'-monophosphate-N-acetylneuraminic acid hydroxylase. Catalytic function and regulation.

Authors: L Shaw; P Schneckenburger; J Carlsen; K Christiansen; R Schauer
Journal: Eur J Biochem Date: 1992-05-15

4. A form of reduced nicotinamide adenine dinucleotide-cytochrome b 5 reductase containing both the catalytic site and an additional hydrophobic membrane-binding segment.

Authors: L Spatz; P Strittmatter
Journal: J Biol Chem Date: 1973-02-10 Impact factor: 5.157

5. Detection of CMP-N-acetylneuraminic acid hydroxylase activity in fractionated mouse liver.

Authors: L Shaw; R Schauer
Journal: Biochem J Date: 1989-10-15 Impact factor: 3.857

6. CMP-N-acetylneuraminic acid hydroxylase activity determines the wheat germ agglutinin-binding phenotype in two mutants of the lymphoma cell line MDAY-D2.

Authors: L Shaw; S Yousefi; J W Dennis; R Schauer
Journal: Glycoconj J Date: 1991-10 Impact factor: 2.916

7. Reconstitution of CMP-N-acetylneuraminic acid hydroxylation activity using a mouse liver cytosol fraction and soluble cytochrome b5 purified from horse erythrocytes.

Authors: Y Kozutsumi; T Kawano; H Kawasaki; K Suzuki; T Yamakawa; A Suzuki
Journal: J Biochem Date: 1991-09 Impact factor: 3.387

8. Characterization of N-glycolylneuraminic acid-containing gangliosides as tumor-associated Hanganutziu-Deicher antigen in human colon cancer.

Authors: H Higashi; Y Hirabayashi; Y Fukui; M Naiki; M Matsumoto; S Ueda; S Kato
Journal: Cancer Res Date: 1985-08 Impact factor: 12.701

Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver.

1. Spectrum of horse-heart cytochrome c.

2. Peroxides in detergents as interfering factors in biochemical analysis.

3. Mouse liver cytidine-5'-monophosphate-N-acetylneuraminic acid hydroxylase. Catalytic function and regulation.

4. A form of reduced nicotinamide adenine dinucleotide-cytochrome b 5 reductase containing both the catalytic site and an additional hydrophobic membrane-binding segment.

5. Detection of CMP-N-acetylneuraminic acid hydroxylase activity in fractionated mouse liver.

6. CMP-N-acetylneuraminic acid hydroxylase activity determines the wheat germ agglutinin-binding phenotype in two mutants of the lymphoma cell line MDAY-D2.

7. Reconstitution of CMP-N-acetylneuraminic acid hydroxylation activity using a mouse liver cytosol fraction and soluble cytochrome b5 purified from horse erythrocytes.

8. Characterization of N-glycolylneuraminic acid-containing gangliosides as tumor-associated Hanganutziu-Deicher antigen in human colon cancer.

9. Developmental sialic acid modifications in rat organs.

10. Autoxidation of soluble trypsin-cleaved microsomal ferrocytochrome b5 and formation of superoxide radicals.

1. A comprehensive phylogenetic analysis of Rieske and Rieske-type iron-sulfur proteins.

Review 2. Exploration of the Sialic Acid World.