Literature DB >> 7841199

Cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from Entamoeba histolytica.

M Huang1, R A Albach, K P Chang, R L Tripathi, R G Kemp.   

Abstract

Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 bp and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved.

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Year:  1995        PMID: 7841199     DOI: 10.1016/0167-4781(94)00216-p

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  5 in total

1.  Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3(2).

Authors:  A M Alves; G J Euverink; M J Bibb; L Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

2.  Evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite Entamoeba histolytica.

Authors:  B Rosenthal; Z Mai; D Caplivski; S Ghosh; H de la Vega; T Graf; J Samuelson
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

3.  Cloning and expression of the gene for the active PPi-dependent phosphofructokinase of Entamoeba histolytica.

Authors:  Z Deng; M Huang; K Singh; R A Albach; S P Latshaw; K P Chang; R G Kemp
Journal:  Biochem J       Date:  1998-02-01       Impact factor: 3.857

4.  Pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica: molecular cloning, recombinant expression and inhibition by pyrophosphate analogues.

Authors:  I Bruchhaus; T Jacobs; M Denart; E Tannich
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

5.  Characterization and phylogeny of the pfp gene of Amycolatopsis methanolica encoding PPi-dependent phosphofructokinase.

Authors:  A M Alves; W G Meijer; J W Vrijbloed; L Dijkhuizen
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

  5 in total

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