Literature DB >> 7840968

Optimization of the transfection of human endothelial cells by electroporation.

J L Schwachtgen1, V Ferreira, D Meyer, D Kerbiriou-Nabias.   

Abstract

A method for efficient transfection of human umbilical vein endothelial cells (HUVEC) is presented here. The procedure involves cell synchronization followed by electroporation and allows the detection of the activity of low-strength promoters. Sodium butyrate, added to the culture medium after electroporation, strongly potentiates the transcriptional activity of viral promoters. The method can be successfully applied to analyze the transcriptional activity of different promoters linked to reporter genes or to express foreign genes controlled by viral promoters.

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Year:  1994        PMID： 7840968

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

Review 1.  What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues.

Authors:  Jean-Michel Escoffre; Thomas Portet; Luc Wasungu; Justin Teissié; David Dean; Marie-Pierre Rols
Journal:  Mol Biotechnol       Date:  2008-11-18       Impact factor: 2.695

Review 2.  Physical non-viral gene delivery methods for tissue engineering.

Authors:  Adam J Mellott; M Laird Forrest; Michael S Detamore
Journal:  Ann Biomed Eng       Date:  2012-10-26       Impact factor: 3.934

3.  ZAP-70 restoration in mice by in vivo thymic electroporation.

Authors:  Magali Irla; Murielle Saade; Adrien Kissenpfennig; Lionel Franz Poulin; Lee Leserman; Patrice N Marche; Evelyne Jouvin-Marche; François Berger; Catherine Nguyen
Journal:  PLoS One       Date:  2008-04-30       Impact factor: 3.240

4.  Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells.

Authors:  L Pasquet; E Bellard; M P Rols; M Golzio; J Teissie
Journal:  Biochem Biophys Rep       Date:  2016-07-17

Review 5.  Gene Electrotransfer: A Mechanistic Perspective.

Authors:  Christelle Rosazza; Sasa Haberl Meglic; Andreas Zumbusch; Marie-Pierre Rols; Damijan Miklavcic
Journal:  Curr Gene Ther       Date:  2016       Impact factor: 4.391
  5 in total

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