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Literature DB >> 7838690

The droplet technique: measurement of calcium extrusion from single isolated mammalian cells.

A V Tepikin¹, J Llopis, V A Snitsarev, D V Gallacher, O H Petersen.

Abstract

This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.

Entities: Chemical Disease Species

Mesh：

Substances：
Calcium

Year: 1994 PMID： 7838690 DOI： 10.1007/bf00374591

Source DB: PubMed Journal: Pflugers Arch ISSN： 0031-6768 Impact factor: 3.657

13 in total

1. Pulsatile Ca2+ extrusion from single pancreatic acinar cells during receptor-activated cytosolic Ca2+ spiking.

Authors: A V Tepikin; S G Voronina; D V Gallacher; O H Petersen
Journal: J Biol Chem Date: 1992-07-15 Impact factor: 5.157

2. Extrusion of calcium from a single isolated neuron of the snail Helix pomatia.

Authors: A V Tepikin; P G Kostyuk; V A Snitsarev; P V Belan
Journal: J Membr Biol Date: 1991-07 Impact factor: 1.843

3. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores.

Authors: A Minta; J P Kao; R Y Tsien
Journal: J Biol Chem Date: 1989-05-15 Impact factor: 5.157

4. Acetylcholine and cholecystokinin induce different patterns of oscillating calcium signals in pancreatic acinar cells.

Authors: D I Yule; A M Lawrie; D V Gallacher
Journal: Cell Calcium Date: 1991 Feb-Mar Impact factor: 6.817

5. A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Authors: G Grynkiewicz; M Poenie; R Y Tsien
Journal: J Biol Chem Date: 1985-03-25 Impact factor: 5.157

6. Ca2+-selective microelectrodes.

Authors: D Ammann
Journal: Cell Calcium Date: 1985-04 Impact factor: 6.817

7. Activation of the plasma membrane Ca2+ pump during agonist stimulation of pancreatic acini.

Authors: B X Zhang; H Zhao; P Loessberg; S Muallem
Journal: J Biol Chem Date: 1992-08-05 Impact factor: 5.157

8. Evidence for agonist-induced export of intracellular Ca2+ in epithelial cells.

Authors: T Wolff; J Leipziger; K G Fischer; B Klär; R Nitschke; R Greger
Journal: Pflugers Arch Date: 1993-09 Impact factor: 3.657

9. Acetylcholine-evoked increase in the cytoplasmic Ca2+ concentration and Ca2+ extrusion measured simultaneously in single mouse pancreatic acinar cells.

Authors: A V Tepikin; S G Voronina; D V Gallacher; O H Petersen
Journal: J Biol Chem Date: 1992-02-25 Impact factor: 5.157

10. Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra.

Authors: M Konishi; S Hollingworth; A B Harkins; S M Baylor
Journal: J Gen Physiol Date: 1991-02 Impact factor: 4.086

7 in total

Review 7. The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.

Authors: Andrea Gerbino; Matilde Colella
Journal: Int J Mol Sci Date: 2018-03-27 Impact factor: 5.923

7 in total

The droplet technique: measurement of calcium extrusion from single isolated mammalian cells.

1. Pulsatile Ca2+ extrusion from single pancreatic acinar cells during receptor-activated cytosolic Ca2+ spiking.

2. Extrusion of calcium from a single isolated neuron of the snail Helix pomatia.

3. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores.

4. Acetylcholine and cholecystokinin induce different patterns of oscillating calcium signals in pancreatic acinar cells.

5. A new generation of Ca2+ indicators with greatly improved fluorescence properties.

6. Ca2+-selective microelectrodes.

7. Activation of the plasma membrane Ca2+ pump during agonist stimulation of pancreatic acini.

8. Evidence for agonist-induced export of intracellular Ca2+ in epithelial cells.

9. Acetylcholine-evoked increase in the cytoplasmic Ca2+ concentration and Ca2+ extrusion measured simultaneously in single mouse pancreatic acinar cells.

10. Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra.

Review 1. Extracellular calcium as an integrator of tissue function.

2. The Physiological Society, proceedings of the scientific meetings of February 1996, March 1996, October 1995.

3. Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells.

4. Asymmetrical, agonist-induced fluctuations in local extracellular [Ca(2+)] in intact polarized epithelia.

5. Calcium dependence of calcium extrusion and calcium uptake in mouse pancreatic acinar cells.

Review 6. Recent advances in understanding the extracellular calcium-sensing receptor.

Review 7. The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.