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Literature DB >> 7833036

Direct PCR of whole blood and hair shafts by microwave treatment.

Abstract

We report a simple and rapid method for DNA preparation suitable for PCR by microwave irradiation. When mouse whole blood and hair shafts were directly irradiated and subjected to PCR, a unique gene such as the retinoblastoma susceptibility gene was reproducibly amplified. This method was useful for screening a transgene sequence in transgenic mice.

Entities: Disease Species

Mesh：

Year: 1994 PMID： 7833036

Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993

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Cited

6 in total

1. Identification of three genetic risk factors for venous thrombosis using a multiplex allele-specific PCR assay: comparison of conventional and new alternative methods for the preparation of DNA from clinical samples.

Authors: Antonio Angelini; Concetta Di Febbo; Giovanna Baccante; Marcello Di Nisio; Carmine Di Ilio; Franco Cuccurullo; Ettore Porreca
Journal: J Thromb Thrombolysis Date: 2003-12 Impact factor: 2.300

2. Adverse effects of vapocoolant and topical anesthesia for tail biopsy of preweanling mice.

Authors: Gillian C Braden; Angela K Brice; F Claire Hankenson
Journal: J Am Assoc Lab Anim Sci Date: 2015-05 Impact factor: 1.232

3. A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault.

Authors: Shanan S Tobe; Yuvaneswari C Swaran; Lynn Dennany; Ursula Sibbing; Kristina Schulze Johann; Lindsey Welch; Marielle Vennemann
Journal: Int J Legal Med Date: 2016-11-10 Impact factor: 2.686

Direct PCR of whole blood and hair shafts by microwave treatment.

1. Identification of three genetic risk factors for venous thrombosis using a multiplex allele-specific PCR assay: comparison of conventional and new alternative methods for the preparation of DNA from clinical samples.

2. Adverse effects of vapocoolant and topical anesthesia for tail biopsy of preweanling mice.

3. A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault.

4. Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously.

5. Mutation in the 3' region of the alpha-1-antitrypsin gene and chronic obstructive pulmonary disease.

6. Nucleic acid purification from plants, animals and microbes in under 30 seconds.