Literature DB >> 7832769

Escherichia coli dihydrodipicolinate synthase: characterization of the imine intermediate and the product of bromopyruvate treatment by electrospray mass spectrometry.

E B Borthwick1, S J Connell, D W Tudor, D J Robins, A Shneier, C Abell, J R Coggins.   

Abstract

Simplified procedures for assaying and purifying dihydrodipicolinate synthase (EC 4.2.1.52), the first enzyme of the lysine biosynthetic pathway, have been developed and electrospray ionization m.s. has been used to observe the imine intermediate with pyruvate and to investigate the reaction of the enzyme with bromopyruvate and fluoropyruvate.

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Year:  1995        PMID： 7832769      PMCID： PMC1136393          DOI： 10.1042/bj3050521

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

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Journal:  J Biol Chem       Date:  1955-03       Impact factor: 5.157

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Authors:  S Bonnassie; J Oreglia; A M Sicard
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

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Authors:  J G Shedlarski; C Gilvarg
Journal:  J Biol Chem       Date:  1970-03-25       Impact factor: 5.157

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Authors:  Y Yugari; C Gilvarg
Journal:  J Biol Chem       Date:  1965-12       Impact factor: 5.157

5.  Molecular cloning of wheat dihydrodipicolinate synthase.

Authors:  T Kaneko; T Hashimoto; R Kumpaisal; Y Yamada
Journal:  J Biol Chem       Date:  1990-10-15       Impact factor: 5.157

6.  Purification and characterization of dihydrodipicolinate synthase from wheat suspension cultures.

Authors:  R Kumpaisal; T Hashimoto; Y Yamada
Journal:  Plant Physiol       Date:  1987-09       Impact factor: 8.340

7.  Direct genetic selection of a maize cDNA for dihydrodipicolinate synthase in an Escherichia coli dapA- auxotroph.

Authors:  D A Frisch; A M Tommey; B G Gengenbach; D A Somers
Journal:  Mol Gen Genet       Date:  1991-08

8.  Isolation of dipicolinic acid (pyridine-2:6-dicarboxylic acid) from spores of Bacillus megatherium.

Authors:  J F POWELL
Journal:  Biochem J       Date:  1953-05       Impact factor: 3.857

9.  Pyridine-2,6-dicarboxylic acid (dipicolinic acid) formation in Bacillus subtilis. I. Non-enzymatic formation of dipicolinic acid from pyruvate and aspartic semialdehyde.

Authors:  K Kimura
Journal:  J Biochem       Date:  1974-05       Impact factor: 3.387

10.  Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization.

Authors:  B Laber; F X Gomis-Rüth; M J Romão; R Huber
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857
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  6 in total

1.  Escherichia coli L-aspartate-alpha-decarboxylase: preprotein processing and observation of reaction intermediates by electrospray mass spectrometry.

Authors:  M K Ramjee; U Genschel; C Abell; A G Smith
Journal:  Biochem J       Date:  1997-05-01       Impact factor: 3.857

2.  Over-expression of GAPDH in human colorectal carcinoma as a preferred target of 3-bromopyruvate propyl ester.

Authors:  Zhenjie Tang; Shuqiang Yuan; Yumin Hu; Hui Zhang; Wenjing Wu; Zhaolei Zeng; Jing Yang; Jingping Yun; Ruihua Xu; Peng Huang
Journal:  J Bioenerg Biomembr       Date:  2012-02-18       Impact factor: 2.945

3.  Kinetic and structural studies of the reaction of Escherichia coli dihydrodipicolinate synthase with (S)-2-bromopropionate.

Authors:  Lilian Chooback; Leonard N Thomas; Nathan Blythe; William Karsten
Journal:  Acta Crystallogr D Struct Biol       Date:  2022-06-08       Impact factor: 5.699

4.  Conserved main-chain peptide distortions: a proposed role for Ile203 in catalysis by dihydrodipicolinate synthase.

Authors:  Renwick C J Dobson; Michael D W Griffin; Sean R A Devenish; F Grant Pearce; Craig A Hutton; Juliet A Gerrard; Geoffrey B Jameson; Matthew A Perugini
Journal:  Protein Sci       Date:  2008-09-11       Impact factor: 6.725

5.  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death.

Authors:  Shanmugasundaram Ganapathy-Kanniappan; Jean-Francois H Geschwind; Rani Kunjithapatham; Manon Buijs; Josephina A Vossen; Irina Tchernyshyov; Robert N Cole; Labiq H Syed; Pramod P Rao; Shinichi Ota; Mustafa Vali
Journal:  Anticancer Res       Date:  2009-12       Impact factor: 2.480

6.  Dihydrodipicolinate synthase is not inhibited by its substrate, (S)-aspartate beta-semialdehyde.

Authors:  Renwick C J Dobson; Juliet A Gerrard; F Grant Pearce
Journal:  Biochem J       Date:  2004-02-01       Impact factor: 3.857
  6 in total

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