| Literature DB >> 782922 |
A J Shatkin, A K Banerjee, G W Both, Y Furuichi, S Muthukrishnan.
Abstract
Many eukaryotic cell and viral mRNAs contain 5'-terminal m7G(5')ppp(5')N. Methylation is important for the in vitro translation of vesicular stomatitis virus and reovirus mRNAs. Unmethylated viral mRNAs are methylated by cell-free extrascts of wheat germ and L cells to form 5'-terminal structures of the type, m7GpppN and m7GpppNm, respectively. Rabbit globin mRNA also contains 5'-terminal 7-methylguanosine (m7G). Removal of the m7G by beta-elimination decreases translation. The efficient binding of mRNA to ribosomes is dependent on the presence of 5'-terminal m7G.Entities:
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Year: 1976 PMID： 782922
Source DB: PubMed Journal: Fed Proc ISSN： 0014-9446