| Literature DB >> 7827297 |
K Okada1, Y Shimizu, S Nambu, K Higuchi, A Watanabe.
Abstract
The tumour cells of a human cholangiocarcinoma cell line, HuCC-T1, were found to express mRNA of interleukin-6 (IL-6) and to secrete a large amount of biologically active IL-6 in the culture medium at the concentration of 22.6 ng/mL. Interleukin-6 was demonstrated in the cytoplasm of the cells by immunohistochemical staining. Furthermore, these cells showed the presence of receptors for IL-6 on the surface, and DNA synthesis of the cells was stimulated by the exogenous addition of recombinant human IL-6 into the culture medium. The cell growth was significantly inhibited in the presence of anti-human IL-6 antibody in the culture medium. These findings indicate that IL-6 is one of the autocrine growth factors of this cell line in vitro.Entities:
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Year: 1994 PMID： 7827297 DOI： 10.1111/j.1440-1746.1994.tb01275.x
Source DB: PubMed Journal: J Gastroenterol Hepatol ISSN： 0815-9319 Impact factor: 4.029