Fluorescence properties of oxidised human plasma low-density lipoproteins.

Appearance of fluorescence emission between 380-550 nm (lambda exc 350-400 nm) in freshly prepared low-density lipoprotein from asymptomatic normolipemic human plasma revealed the presence of in vivo oxidative modification of its protein moiety. Low-density lipoprotein elicited seven fluorophores in three dimensional fluorescence spectrogram. Assignment of fluorescent chemical structures originating from oxidative modification of the protein moiety of low-density lipoprotein has been made with the help of second derivative fluorescence spectroscopy.