OBJECTIVES: Excessive formation of reactive oxygen species (ROS) in human semen has been associated with impaired sperm function and fertility potential. The presence of ROS in semen specimens from normal fertile men emphasizes the importance of defining a normal range of ROS formation. The purpose of this study was to establish a normal range of ROS generation and to investigate the effect of sperm concentration on the ROS level. METHODS: ROS was determined in 15 healthy donors and 20 men with suspected infertility. After the sperm concentration in normal donors was adjusted to 20 x 10(6)/mL, ROS was measured by chemiluminescence using luminol in a Berthold luminometer. A specimen was regarded as positive (abnormal) when the value was at least 10 x 10(4) counts per minute (cpm). ROS was also evaluated at 4 sperm concentrations (60, 30, 15, and 7.5 x 10(6)/mL) from samples obtained from the patients with suspected infertility. In addition, ROS was measured in 7 ROS-positive specimens at a sperm concentration of 15 x 10(6)/mL and 60 x 10(6)/mL. RESULTS: Results showed that ROS formation was negative in all 15 healthy donors (median, 0.9 x 10(4) cpm; interquartile range, 0 to 1.48 x 10(4) cpm). The ROS formation value among all the donors was less than 5.5 x 10(4) cpm. ROS formation was positive in 8 (40%) of the suspected infertile patients. ROS levels were significantly lower at sperm concentrations of 15 x 10(6)/mL or 7.5 x 10(6)/mL compared with 30 x 10(6)/mL or 60 x 10(6)/mL (P = 0.05). The ROS level increased after centrifugation for 10 minutes at 500 g in all 7 specimens at both 15 and 60 x 10(6)/mL. However, the increase in ROS formation at 60 x 10(6)/mL was significantly greater than that at 15 x 10(6)/mL (P < 0.001). CONCLUSIONS: A range of ROS formation of 0 to 5.5 x 10(4) cpm at a sperm concentration of 20 x 10(6)/mL may be considered as normal for healthy donor semen. The positive relationship between ROS formation and sperm concentration at the time of measurement emphasizes the importance of concentration adjustment before analysis when comparing ROS levels between different specimens.
OBJECTIVES: Excessive formation of reactive oxygen species (ROS) in human semen has been associated with impaired sperm function and fertility potential. The presence of ROS in semen specimens from normal fertile men emphasizes the importance of defining a normal range of ROS formation. The purpose of this study was to establish a normal range of ROS generation and to investigate the effect of sperm concentration on the ROS level. METHODS:ROS was determined in 15 healthy donors and 20 men with suspected infertility. After the sperm concentration in normal donors was adjusted to 20 x 10(6)/mL, ROS was measured by chemiluminescence using luminol in a Berthold luminometer. A specimen was regarded as positive (abnormal) when the value was at least 10 x 10(4) counts per minute (cpm). ROS was also evaluated at 4 sperm concentrations (60, 30, 15, and 7.5 x 10(6)/mL) from samples obtained from the patients with suspected infertility. In addition, ROS was measured in 7 ROS-positive specimens at a sperm concentration of 15 x 10(6)/mL and 60 x 10(6)/mL. RESULTS: Results showed that ROS formation was negative in all 15 healthy donors (median, 0.9 x 10(4) cpm; interquartile range, 0 to 1.48 x 10(4) cpm). The ROS formation value among all the donors was less than 5.5 x 10(4) cpm. ROS formation was positive in 8 (40%) of the suspected infertilepatients. ROS levels were significantly lower at sperm concentrations of 15 x 10(6)/mL or 7.5 x 10(6)/mL compared with 30 x 10(6)/mL or 60 x 10(6)/mL (P = 0.05). The ROS level increased after centrifugation for 10 minutes at 500 g in all 7 specimens at both 15 and 60 x 10(6)/mL. However, the increase in ROS formation at 60 x 10(6)/mL was significantly greater than that at 15 x 10(6)/mL (P < 0.001). CONCLUSIONS: A range of ROS formation of 0 to 5.5 x 10(4) cpm at a sperm concentration of 20 x 10(6)/mL may be considered as normal for healthy donor semen. The positive relationship between ROS formation and sperm concentration at the time of measurement emphasizes the importance of concentration adjustment before analysis when comparing ROS levels between different specimens.
Authors: Wiep de Ligny; Roos M Smits; Rebecca Mackenzie-Proctor; Vanessa Jordan; Kathrin Fleischer; Jan Peter de Bruin; Marian G Showell Journal: Cochrane Database Syst Rev Date: 2022-05-04
Authors: Roos M Smits; Rebecca Mackenzie-Proctor; Anusch Yazdani; Marcin T Stankiewicz; Vanessa Jordan; Marian G Showell Journal: Cochrane Database Syst Rev Date: 2019-03-14
Authors: Ashok Agarwal; Neel Parekh; Manesh Kumar Panner Selvam; Ralf Henkel; Rupin Shah; Sheryl T Homa; Ranjith Ramasamy; Edmund Ko; Kelton Tremellen; Sandro Esteves; Ahmad Majzoub; Juan G Alvarez; David K Gardner; Channa N Jayasena; Jonathan W Ramsay; Chak Lam Cho; Ramadan Saleh; Denny Sakkas; James M Hotaling; Scott D Lundy; Sarah Vij; Joel Marmar; Jaime Gosalvez; Edmund Sabanegh; Hyun Jun Park; Armand Zini; Parviz Kavoussi; Sava Micic; Ryan Smith; Gian Maria Busetto; Mustafa Emre Bakırcıoğlu; Gerhard Haidl; Giancarlo Balercia; Nicolás Garrido Puchalt; Moncef Ben-Khalifa; Nicholas Tadros; Jackson Kirkman-Browne; Sergey Moskovtsev; Xuefeng Huang; Edson Borges; Daniel Franken; Natan Bar-Chama; Yoshiharu Morimoto; Kazuhisa Tomita; Vasan Satya Srini; Willem Ombelet; Elisabetta Baldi; Monica Muratori; Yasushi Yumura; Sandro La Vignera; Raghavender Kosgi; Marlon P Martinez; Donald P Evenson; Daniel Suslik Zylbersztejn; Matheus Roque; Marcello Cocuzza; Marcelo Vieira; Assaf Ben-Meir; Raoul Orvieto; Eliahu Levitas; Amir Wiser; Mohamed Arafa; Vineet Malhotra; Sijo Joseph Parekattil; Haitham Elbardisi; Luiz Carvalho; Rima Dada; Christophe Sifer; Pankaj Talwar; Ahmet Gudeloglu; Ahmed M A Mahmoud; Khaled Terras; Chadi Yazbeck; Bojanic Nebojsa; Damayanthi Durairajanayagam; Ajina Mounir; Linda G Kahn; Saradha Baskaran; Rishma Dhillon Pai; Donatella Paoli; Kristian Leisegang; Mohamed Reza Moein; Sonia Malik; Onder Yaman; Luna Samanta; Fouad Bayane; Sunil K Jindal; Muammer Kendirci; Baris Altay; Dragoljub Perovic; Avi Harlev Journal: World J Mens Health Date: 2019-05-28 Impact factor: 5.400