Modulation of the gating of the transient outward potassium current of rat isolated cerebellar granule neurons by lanthanum.

The effects of the trivalent cation, lanthanum (La3+) on voltage-dependent K+ conductances were studied in rat isolated cerebellar granule neurons under whole-cell voltage-clamp conditions. La3+ at low micromolar concentrations caused a pronounced enhancement in the outward current evoked by depolarising steps from -50 mV, with the apparent recruitment of an inactivating component. The steady-state inactivation curve for the transient outward current, evoked by depolarising steps from -140 mV, was shifted by approximately 40 mV in the depolarising direction by 10 microM La3+, with a slight increase in the slope factor. The kinetics of activation and inactivation were slowed in the presence of La3+. A shift of 10 mV in the depolarising direction was seen for the activation curve of the delayed rectifier current in the presence of 10 microM La3+. These results indicate that La3+ has a potent effect on the gating characteristics of voltage-activated K+ currents. This effect cannot be explained by surface charge considerations.