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Literature DB >> 7814504

Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.

G A Storch¹, M Gaudreault-Keener, P C Welby.

Abstract

The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.

Entities: Chemical

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Year: 1994 PMID： 7814504 PMCID： PMC264108 DOI： 10.1128/jcm.32.10.2581-2583.1994

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

10 in total

Review 1. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction.

Authors: T H The; M van der Ploeg; A P van den Berg; A M Vlieger; M van der Giessen; W J van Son
Journal: Transplantation Date: 1992-08 Impact factor: 4.939

2. Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood.

Authors: S Izraeli; C Pfleiderer; T Lion
Journal: Nucleic Acids Res Date: 1991-11-11 Impact factor: 16.971

3. Interference of heparin with the polymerase chain reaction.

Authors: E Beutler; T Gelbart; W Kuhl
Journal: Biotechniques Date: 1990-08 Impact factor: 1.993

4. Plasma collected from heparinized blood is not suitable for HCV-RNA detection by conventional RT-PCR assay.

Authors: M Willems; H Moshage; F Nevens; J Fevery; S H Yap
Journal: J Virol Methods Date: 1993-04 Impact factor: 2.014

5. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.

Authors: C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal: J Clin Microbiol Date: 1985-02 Impact factor: 5.948

6. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.

Authors: G A Storch; R S Buller; T C Bailey; N A Ettinger; T Langlois; M Gaudreault-Keener; P L Welby
Journal: J Clin Microbiol Date: 1994-04 Impact factor: 5.948

7. Detection of cytomegalovirus DNA in peripheral blood of patients infected with human immunodeficiency virus.

Authors: D Shibata; W J Martin; M D Appleman; D M Causey; J M Leedom; N Arnheim
Journal: J Infect Dis Date: 1988-12 Impact factor: 5.226

8. Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction.

Authors: N M Jiwa; G W Van Gemert; A K Raap; F M Van de Rijke; A Mulder; P F Lens; M M Salimans; F E Zwaan; W Van Dorp; M Van der Ploeg
Journal: Transplantation Date: 1989-07 Impact factor: 4.939

9. Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients.

Authors: R S Buller; T C Bailey; N A Ettinger; M Keener; T Langlois; J P Miller; G A Storch
Journal: J Clin Microbiol Date: 1992-10 Impact factor: 5.948

10. Inhibition of human immunodeficiency virus gene amplification by heparin.

Authors: M Holodniy; S Kim; D Katzenstein; M Konrad; E Groves; T C Merigan
Journal: J Clin Microbiol Date: 1991-04 Impact factor: 5.948

10 in total

5 in total

1. Anticoagulants interfere with PCR used to diagnose invasive aspergillosis.

Authors: Marta E García; Jose L Blanco; Jesús Caballero; Domingo Gargallo-Viola
Journal: J Clin Microbiol Date: 2002-04 Impact factor: 5.948

2. Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.

Authors: M L Landry; S Cohen; K Huber
Journal: J Clin Microbiol Date: 1997-01 Impact factor: 5.948