Literature DB >> 7813704

Characterization and protective effect of a 29 kDa protein isolated from Coxiella burnetii by detergent Empigen BB.

M Lukácová1, E Gajdosová, L Skultéty, E Kovácová, J Kazár.   

Abstract

A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates a strong binding between proteins and LPS in the outer membrane of C. burnetii. The protein was immunogenic in mice and protected them against virulent C. burnetii challenge.

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Year:  1994        PMID： 7813704     DOI： 10.1007/bf01730376

Source DB:  PubMed          Journal:  Eur J Epidemiol        ISSN： 0393-2990            Impact factor:   8.082


  21 in total

1.  A method of purifying Coxiella burnetii and other pathogenic Rickettsiae.

Authors:  R A ORMSBEE
Journal:  J Immunol       Date:  1962-01       Impact factor: 5.422

2.  Chemical composition of phase I Coxiella burnetii soluble antigen prepared by trichloroacetic acid extraction.

Authors:  M Lukácová; R Brezina; S Schramek; J Pastorek
Journal:  Acta Virol       Date:  1989-01       Impact factor: 1.162

3.  Steric hindrance of antibody binding to surface proteins of Coxiella burnetti by phase I lipopolysaccharide.

Authors:  T Hackstadt
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

4.  Immunological properties of the lipopolysaccharide-protein complex of Coxiella burnetii.

Authors:  J Kazár; S Schramek; R Brezina
Journal:  Acta Virol       Date:  1978-07       Impact factor: 1.162

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Isolation of a protein antigen from Coxiella burnetii.

Authors:  H P Müller; N Schmeer; L Rantamäki; B Semler; H Krauss
Journal:  Zentralbl Bakteriol Mikrobiol Hyg A       Date:  1987-07

8.  Monoclonal antibodies distinguish phase variants of Coxiella burnetii.

Authors:  J C Williams; M R Johnston; M G Peacock; L A Thomas; S Stewart; J L Portis
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441

9.  Antigenic differences between Coxiella burnetii cells revealed by postembedding immunoelectron microscopy and immunoblotting.

Authors:  T F McCaul; N Banerjee-Bhatnagar; J C Williams
Journal:  Infect Immun       Date:  1991-09       Impact factor: 3.441

10.  Peptides bound to proteosomes via hydrophobic feet become highly immunogenic without adjuvants.

Authors:  G H Lowell; L F Smith; R C Seid; W D Zollinger
Journal:  J Exp Med       Date:  1988-02-01       Impact factor: 14.307
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  3 in total

1.  Cloning and porin activity of the major outer membrane protein P1 from Coxiella burnetii.

Authors:  Sunita Varghees; Kati Kiss; Giovanni Frans; Orit Braha; James E Samuel
Journal:  Infect Immun       Date:  2002-12       Impact factor: 3.441

2.  Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane protein.

Authors:  Q Zhang; J C Meitzler; S Huang; T Morishita
Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

3.  Identification and cloning of immunodominant antigens of Coxiella burnetii.

Authors:  Guoquan Zhang; Katalin Kiss; Rekha Seshadri; Laura R Hendrix; James E Samuel
Journal:  Infect Immun       Date:  2004-02       Impact factor: 3.441
  3 in total

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