Literature DB >> 7812625

The identification of apparently novel cyclic AMP and cyclic GMP phosphodiesterase activities in guinea-pig tracheal smooth muscle.

F Burns1, P A Stevens, N J Pyne.   

Abstract

Phosphodiesterase (PDE) activities that were capable of hydrolysing cyclic AMP (Km = 6.8 +/- 2 microM) and cyclic GMP (Km = 6.7 +/- 1.6 microM) were isolated from tracheal smooth muscle. These enzyme(s) activities were insensitive to stimulation by calcium/calmodulin and to inhibition by cyclic GMP, rolipram (type IV inhibitor) and siguazodan (type III inhibitor). Zaprinast was a relatively poor inhibitor of both cyclic AMP and cyclic GMP hydrolysis (IC50 = 46 +/- 9 microM and 45 +/- 14 microM respectively). These results suggest that tracheal smooth muscle may contain an apparently novel PDE. However, KCl (30 mM) which facilitates calcium entry in cells, depressed bradykinin-stimulated intracellular cyclic AMP formation, suggesting that the type I PDE may be functionally present. We suggest that considerable caution be exercised in identifying apparently novel PDE isoforms.

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Year:  1994        PMID： 7812625      PMCID： PMC1510075          DOI： 10.1111/j.1476-5381.1994.tb16164.x

Source DB:  PubMed          Journal:  Br J Pharmacol        ISSN： 0007-1188            Impact factor:   8.739


  5 in total

1.  Proteolysis of cyclic AMP phosphodiesterase-II attenuates its ability to be inhibited by compounds which exert positive inotropic actions in cardiac tissue.

Authors:  B Price; N J Pyne; M D Houslay
Journal:  Biochem Pharmacol       Date:  1987-12-01       Impact factor: 5.858

2.  Characterization and selective inhibition of cyclic nucleotide phosphodiesterase isozymes in canine tracheal smooth muscle.

Authors:  T J Torphy; L B Cieslinski
Journal:  Mol Pharmacol       Date:  1990-02       Impact factor: 4.436

3.  Separation and characterization of a novel isoenzyme of cyclic nucleotide phosphodiesterase from rat cerebrum.

Authors:  J Mukai; T Asai; M Naka; T Tanaka
Journal:  Br J Pharmacol       Date:  1994-02       Impact factor: 8.739

4.  Interaction of the catalytic subunit of protein kinase A with the lung type V cyclic GMP phosphodiesterase: modulation of non-catalytic binding sites.

Authors:  F Burns; N J Pyne
Journal:  Biochem Biophys Res Commun       Date:  1992-12-30       Impact factor: 3.575

5.  Bradykinin stimulates phospholipase D in primary cultures of guinea-pig tracheal smooth muscle.

Authors:  S Pyne; N J Pyne
Journal:  Biochem Pharmacol       Date:  1993-02-09       Impact factor: 5.858
  5 in total

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