OBJECTIVE: To find out if the opioids that are liberated during bleeding influence the concentrations of glucagon, insulin, somatostatin, corticosterone and glucose in rats, and if naloxone has any regulatory effect. DESIGN: Laboratory study. SETTING: University hospital. MATERIAL: 56 Male Wistar rats. INTERVENTIONS: Removal of blood corresponding to 2% of body weight through a catheter in the internal jugular vein. Before bleeding (n = 8), and at 5, 15 and 30 minutes afterwards (n = 16 at each time point, half of which had received naloxone 0.04 mg/ml, 0.7 ml/hour, for 10 minutes before bleeding and up to 30 minutes afterwards) rats were killed and samples of heart blood taken. MAIN OUTCOME MEASURES: Concentrations of glucose, glucagon, insulin, somatostatin, and corticosterone in heart blood. RESULTS: In the control group (saline) concentrations of glucose, glucagon and insulin increased significantly after bleeding, and had returned to baseline concentration by 30 minutes. Naloxone blocked the increases in the treated group. CONCLUSION: Endogenous opioids, possibly endorphin, may be important in the acute regulation of blood glucose, glucagon and insulin concentrations after haemorrhage.
OBJECTIVE: To find out if the opioids that are liberated during bleeding influence the concentrations of glucagon, insulin, somatostatin, corticosterone and glucose in rats, and if naloxone has any regulatory effect. DESIGN: Laboratory study. SETTING: University hospital. MATERIAL: 56 Male Wistar rats. INTERVENTIONS: Removal of blood corresponding to 2% of body weight through a catheter in the internal jugular vein. Before bleeding (n = 8), and at 5, 15 and 30 minutes afterwards (n = 16 at each time point, half of which had received naloxone 0.04 mg/ml, 0.7 ml/hour, for 10 minutes before bleeding and up to 30 minutes afterwards) rats were killed and samples of heart blood taken. MAIN OUTCOME MEASURES: Concentrations of glucose, glucagon, insulin, somatostatin, and corticosterone in heart blood. RESULTS: In the control group (saline) concentrations of glucose, glucagon and insulin increased significantly after bleeding, and had returned to baseline concentration by 30 minutes. Naloxone blocked the increases in the treated group. CONCLUSION: Endogenous opioids, possibly endorphin, may be important in the acute regulation of blood glucose, glucagon and insulin concentrations after haemorrhage.
Authors: James R Gavin; Rolf Kubin; Shurjeel Choudhri; Dagmar Kubitza; Hebert Himmel; Rainer Gross; Jutta M Meyer Journal: Drug Saf Date: 2004 Impact factor: 5.606