OBJECTIVE: To determine the possible effect of freezing on sperm escape force as measured by laser trap. DESIGN: Controlled clinical study. SETTING: Normal volunteers, academic setting. PATIENTS: Normal, healthy volunteers. INTERVENTION: Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force before and after freezing. MAIN OUTCOME MEASURE: Comparison of escape force as a measurement of freezing effects. RESULTS: Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh (76.1 +/- 31.1 mW) and in frozen-thawed samples (75.6 +/- 40.0 mW) were similar. CONCLUSION: Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.
OBJECTIVE: To determine the possible effect of freezing on sperm escape force as measured by laser trap. DESIGN: Controlled clinical study. SETTING: Normal volunteers, academic setting. PATIENTS: Normal, healthy volunteers. INTERVENTION: Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force before and after freezing. MAIN OUTCOME MEASURE: Comparison of escape force as a measurement of freezing effects. RESULTS: Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh (76.1 +/- 31.1 mW) and in frozen-thawed samples (75.6 +/- 40.0 mW) were similar. CONCLUSION: Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.
Authors: Jaclyn M Nascimento; Linda Z Shi; Stuart Meyers; Pascal Gagneux; Naida M Loskutoff; Elliot L Botvinick; Michael W Berns Journal: J R Soc Interface Date: 2008-03-06 Impact factor: 4.118
Authors: Jaclyn M Nascimento; Linda Z Shi; Charlie Chandsawangbhuwana; James Tam; Barbara Durrant; Elliot L Botvinick; Michael W Berns Journal: J Biomed Opt Date: 2008 Jan-Feb Impact factor: 3.170
Authors: Linda Z Shi; Jaclyn M Nascimento; Charlie Chandsawangbhuwana; Elliot L Botvinick; Michael W Berns Journal: Biomed Microdevices Date: 2008-08 Impact factor: 2.838
Authors: Nicole Auka; Michael Valle; Bobby D Cox; Peter D Wilkerson; Tracey Dawson Cruz; Joseph E Reiner; Sarah J Seashols-Williams Journal: PLoS One Date: 2019-02-07 Impact factor: 3.240