Literature DB >> 780373

Preparation of rabies fluorescein isothiocyanate-labeled immune globulin from mouse hyperimmune ascitic fluids.

T Tzianabos, G A Hebert, L A White.   

Abstract

Immunization conditions for the production of mouse immune ascitic fluids to be used for the preparation of rabies fluorescent antibody (FA) conjugate are presented. The use of optimal concentrations of ammonium sulfate for precipitation of gamma globulin resulted in a fraction consisting of 75% gamma globulin and 25% alpha-beta globulins with no detectable albumin. Dialysis labeling of the globulin fration with fluorescein isothiocyanate produced a specific rabies FA conjugate with negligible nonspecific background staining. This procedure represents a simple means of producing rabies FA conjugate.

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Year:  1976        PMID： 780373      PMCID： PMC274364          DOI： 10.1128/jcm.3.6.609-612.1976

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY.

Authors:  H F CLARK; C C SHEPARD
Journal:  Virology       Date:  1963-08       Impact factor: 3.616

2.  THE DIAGNOSIS OF RABIES BY FLUORESCENT ANTIBODY METHOD (FRA) EMPLOYING IMMUNE HAMSTER SERUM.

Authors:  E H LENNETTE; J D WOODIE; K NAKAMURA; R L MAGOFFIN
Journal:  Health Lab Sci       Date:  1965-01

3.  Fluorescent antibody staining of rabies virus antigens in the salivary glands of rabid animals.

Authors:  R A GOLDWASSER; R E KISSLING; T R CARSKI; T S HOSTY
Journal:  Bull World Health Organ       Date:  1959       Impact factor: 9.408

4.  Fluorescent antibody staining of street and fixed rabies virus antigens.

Authors:  R A GOLDWASSER; R E KISSLING
Journal:  Proc Soc Exp Biol Med       Date:  1958-06

5.  [The preparation of rabies virus nucleocapsid specific IgG and its purification and conjugation with peroxidase or fluorescein isothiocyanate (author's transl)].

Authors:  P Atanasiu; H Tsiang; J Virat
Journal:  Ann Microbiol (Paris)       Date:  1974 Jul-Aug

6.  The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses.

Authors:  E S Tikasingh; L Spence; W G Downs
Journal:  Am J Trop Med Hyg       Date:  1966-03       Impact factor: 2.345

7.  Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse.

Authors:  A C Sartorelli; D S Fischer; W G Downs
Journal:  J Immunol       Date:  1966-04       Impact factor: 5.422

8.  Ammonium sulfate fractionation of sera: mouse, hamster, guinea pig, monkey, chimpanzee, swine, chicken, and cattle.

Authors:  G A Hebert
Journal:  Appl Microbiol       Date:  1974-02

9.  Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.

Authors:  G A Hebert; P L Pelham; B Pittman
Journal:  Appl Microbiol       Date:  1973-01
  9 in total
  1 in total

1.  Optimal fluorescein-to-protein ratios of bacterial direct fluorescent-antibody reagents.

Authors:  G A Hébert; B Pittman; R M McKinney
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948
  1 in total

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