Actin-bound nucleotide/divalent cation interactions.

At this point, it may be worthwhile to list, in summary form, the important aspects of divalent cation and nucleotide binding to actin that have been reviewed here: 1) High affinity divalent cation binding to actin is very tight, with equilibrium dissociation constant KCa approximately 1 nM and KMg approximately 5 nM at pH 7.0. 2) The binding kinetics of Ca++ are diffusion limited. Dissociation is slow, with k-Ca approximately 0.015 sec at pH 7.0 (and low ionic strength). 3) The binding kinetics of Mg++ are limited by the characteristics of the Mg++ aquo-ion and are much slower than for Ca++; k-Mg approximately 0.0012 at pH 7.0. 4) Increase in pH or ionic strength weakens divalent cation binding at the high affinity site, primarily by increasing k-Ca and k-Mg. 5) Exchange of Mg++ for Ca++ (or vice versa) at the high affinity site is by a competitive pseudo-first order process with an apparent rate constant (kapp) intermediate between k-Ca and k-Mg and dependent upon the cation concentration ratio [Ca]/[Mg] present. 6) High affinity ATP binding is modulated by the high affinity divalent cation. The cation concentration range over which this modulation occurs is about 100-fold higher for Mg++ than for Ca++, again because of the different characteristics of the Mg++ and Ca++ aquo-ions. 7) At low divalent cation concentrations, ATP dissociation from actin is limited by dissociation of the tightly-bound divalent cation. 8) At high divalent cation concentrations, ATP dissociation probably occurs via dissociation of the divalent cation-nucleotide complex and is quite slow, with dissociation rate constant approximately 0.0005 sec-1. 9) Competitive nucleotide exchange on actin may be described by a pseudo-first order model analogous to that for divalent cation exchange. The pseudo-first order rate constants depend upon the divalent cation concentration. The overall nucleotide exchange rate constant kex depends upon these constants and the solution nucleotide concentration ratio, e.g. [ATP]/[ADP]. The following circumstances develop from the characteristics of the high affinity binding of divalent cation and nucleotide to actin: 1) The standard methods for actin preparation convert in vivo Mg-actin into Ca-actin. 2) Converting Ca-actin back to Mg-actin is not easy. A very low ratio of [Ca]/[Mg] is necessary, which usually requires the use of Ca-cheltors, and a long time (5-10 min) must be allowed for complete exchange.(ABSTRACT TRUNCATED AT 400 WORDS)