Literature DB >> 7799145

Cultivation of Leishmania braziliensis in an economical serum-free medium containing human urine.

T C Armstrong1, J L Patterson.   

Abstract

Leishmania braziliensis cells are difficult to culture in vitro and usually require media supplemented with serum for sustained cell division. Fresh, sterile urine is an inexpensive substitute for serum in the culture of 2 strains of L. braziliensis, 1 infected with Leishmania RNA virus 1, and 1 uninfected. In the presence of urine, both the infected and the uninfected strains grew to the same final cell density as the same strains grown in the presence of serum. One strain of Leishmania major was also successfully cultured in urine-supplemented media.

Entities:  

Mesh:

Substances:

Year:  1994        PMID: 7799145

Source DB:  PubMed          Journal:  J Parasitol        ISSN: 0022-3395            Impact factor:   1.276


  10 in total

1.  Identification of the minimal essential RNA sequences responsible for site-specific targeting of the Leishmania RNA virus 1-4 capsid endoribonuclease.

Authors:  Y T Ro; J L Patterson
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

2.  Detection of Leishmania (Leishmania) infantum RNA in fleas and ticks collected from naturally infected dogs.

Authors:  Fabio A Colombo; Rosa M F N Odorizzi; Marcia D Laurenti; Eunice A B Galati; Flavio Canavez; Vera L Pereira-Chioccola
Journal:  Parasitol Res       Date:  2011-01-11       Impact factor: 2.289

3.  LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of Leishmania major.

Authors:  Vahid Nasiri; Abdolhossein Dalimi; Fatemeh Ghaffarifar
Journal:  J Parasit Dis       Date:  2016-05-26

4.  Concentration of 2'C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase.

Authors:  John I Robinson; Stephen M Beverley
Journal:  J Biol Chem       Date:  2018-03-06       Impact factor: 5.157

5.  Single-site cleavage in the 5'-untranslated region of Leishmaniavirus RNA is mediated by the viral capsid protein.

Authors:  K J MacBeth; J L Patterson
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-12       Impact factor: 11.205

6.  Low-cost liquid medium for in vitro cultivation of Leishmania parasites in low-income countries.

Authors:  Geremew Tasew; Amha Kebede; Dawit Wolday; Endalamaw Gadisa; Sven Britton; Liv Eidsmo; Hannah Akuffo
Journal:  Glob Health Action       Date:  2009-10-22       Impact factor: 2.640

7.  Milk of cow (Bos taurus), buffalo (Bubalus bubalis), and goat (Capra hircus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation, and maintenance of Leishmania donovani promastigotes.

Authors:  M Muniaraj; C S Lal; S Kumar; P K Sinha; P Das
Journal:  J Clin Microbiol       Date:  2007-02-07       Impact factor: 5.948

Review 8.  Cultivation of clinically significant hemoflagellates.

Authors:  Frederick L Schuster; James J Sullivan
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

9.  Human urine stimulates in vitro growth of Trypanosoma cruzi and Trypanosoma rangeli.

Authors:  Keila A M Ferreira; Paulo E S Lemos-Júnior; Eliane Lages-Silva; Luis E Ramírez; André L Pedrosa
Journal:  Parasitol Res       Date:  2007-07-15       Impact factor: 2.289

10.  Effects of sheep and mouse urine on the growth pattern of Leishmania major promastigotes.

Authors:  Vahid Nasiri; Gholamreza Karimi; Abdolhossein Dalimi; Habibollah Paykari; Fatemeh Ghaffarifar
Journal:  Biomed Res Int       Date:  2013-07-15       Impact factor: 3.411

  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.