Literature DB >> 779741

Rapid detection and isolation of mycoplasmas from cell cultures.

G A Tomkins, A MacGregor, D Pye, M Atkinson.   

Abstract

An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.

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Year:  1975        PMID： 779741     DOI： 10.1038/icb.1975.28

Source DB:  PubMed          Journal:  Aust J Exp Biol Med Sci        ISSN： 0004-945X


  7 in total

1.  Reduced lysyl oxidase activity in skin fibroblasts from patients with Menkes' syndrome.

Authors:  P M Royce; J Camakaris; D M Danks
Journal:  Biochem J       Date:  1980-11-15       Impact factor: 3.857

2.  Normal hydroxylation of proline in collagen synthesized by skin fibroblasts from a patient with prolidase deficiency.

Authors:  P M Royce; D M Danks
Journal:  J Inherit Metab Dis       Date:  1982       Impact factor: 4.982

3.  Some in-vitro comparisons of synovial cells dispersed by trypsin from rheumatoid and nonrheumatoid synovium.

Authors:  B J Clarris; J R Fraser; J F Stanley
Journal:  Ann Rheum Dis       Date:  1981-06       Impact factor: 19.103

4.  Genetics of mammalian phenylalanine hydroxylase system. IV. Evidence of phenylalanine hydroxylase in a cultured human hepatoma cell line.

Authors:  K H Choo; R G Cotton; I G Jennings; K Fowler; D M Danks
Journal:  Biochem Genet       Date:  1980-10       Impact factor: 1.890

5.  Purine synthesis de novo in cultured lymphoblast cells derived from patients with gout.

Authors:  R B Gordon; B T Emmerson
Journal:  Rheumatol Int       Date:  1987       Impact factor: 2.631

6.  Effect of several inhibitors of enzymatic DNA methylation on the in vivo methylation of different classes of DNA sequences in a cultured human cell line.

Authors:  D M Woodcock; J K Adams; R G Allan; I A Cooper
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

7.  Two-dimensional polyacrylamide gel analysis of fibroblast polypeptides: discussion of its relevance for inherited diseases.

Authors:  K H Choo; R G Cotton; D M Danks; U Norris
Journal:  J Inherit Metab Dis       Date:  1981       Impact factor: 4.982
  7 in total

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