Literature DB >> 7789531

Cloning and disruption of the YNR1 gene encoding the nitrate reductase apoenzyme of the yeast Hansenula polymorpha.

J Avila1, M D Pérez, N Brito, C González, J M Siverio.   

Abstract

The nitrate reductase gene (YNR1) from the yeast H. polymorpha was isolated from a lambda EMBL3 genomic DNA library. As probe a 350 bp DNA fragment synthesized by PCR from H. polymorpha cDNA was used. By DNA sequencing an ORF of 2,577 bp was found. The predicted protein has 859 amino acids and presents high identity with nitrate reductases from other organisms. Chromosomal disruption of YNR1 causes inability to grow in nitrate. Northern blot analysis showed that YNR1 expression is induced by nitrate and repressed by ammonium.

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Year:  1995        PMID： 7789531     DOI： 10.1016/0014-5793(95)00511-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  14 in total

1.  The genes YNI1 and YNR1, encoding nitrite reductase and nitrate reductase respectively in the yeast Hansenula polymorpha, are clustered and co-ordinately regulated.

Authors:  N Brito; J Avila; M D Perez; C Gonzalez; J M Siverio
Journal:  Biochem J       Date:  1996-07-01       Impact factor: 3.857

2.  Clustering of the YNA1 gene encoding a Zn(II)2Cys6 transcriptional factor in the yeast Hansenula polymorpha with the nitrate assimilation genes YNT1, YNI1 and YNR1, and its involvement in their transcriptional activation.

Authors:  J Avila; C González; N Brito; J M Siverio
Journal:  Biochem J       Date:  1998-11-01       Impact factor: 3.857

3.  The YNT1 gene encoding the nitrate transporter in the yeast Hansenula polymorpha is clustered with genes YNI1 and YNR1 encoding nitrite reductase and nitrate reductase, and its disruption causes inability to grow in nitrate.

Authors:  M D Pérez; C González; J Avila; N Brito; J M Siverio
Journal:  Biochem J       Date:  1997-01-15       Impact factor: 3.857

4.  Assessing genetic diversity among Brettanomyces yeasts by DNA fingerprinting and whole-genome sequencing.

Authors:  Sam Crauwels; Bo Zhu; Jan Steensels; Pieter Busschaert; Gorik De Samblanx; Kathleen Marchal; Kris A Willems; Kevin J Verstrepen; Bart Lievens
Journal:  Appl Environ Microbiol       Date:  2014-05-09       Impact factor: 4.792

5.  Npr1 Ser/Thr protein kinase links nitrogen source quality and carbon availability with the yeast nitrate transporter (Ynt1) levels.

Authors:  Yusé Martín; Yelvis V González; Elisa Cabrera; Celia Rodríguez; José M Siverio
Journal:  J Biol Chem       Date:  2011-06-07       Impact factor: 5.157

6.  Ure2 is involved in nitrogen catabolite repression and salt tolerance via Ca2+ homeostasis and calcineurin activation in the yeast Hansenula polymorpha.

Authors:  Celia Rodríguez; Paula Tejera; Braulio Medina; Rosa Guillén; Angel Domínguez; José Ramos; José M Siverio
Journal:  J Biol Chem       Date:  2010-09-29       Impact factor: 5.157

7.  Phylogenetic analysis of proteins associated in the four major energy metabolism systems: photosynthesis, aerobic respiration, denitrification, and sulfur respiration.

Authors:  Takeshi Tomiki; Naruya Saitou
Journal:  J Mol Evol       Date:  2004-08       Impact factor: 2.395

8.  Utilization of nitrate abolishes the "Custers effect" in Dekkera bruxellensis and determines a different pattern of fermentation products.

Authors:  Silvia Galafassi; Claudia Capusoni; Md Moktaduzzaman; Concetta Compagno
Journal:  J Ind Microbiol Biotechnol       Date:  2013-01-25       Impact factor: 3.346

9.  Tobacco Nia2 cDNA functionally complements a Hansenula polymorpha yeast mutant lacking nitrate reductase. A new expression system for the study of plant proteins involved in nitrate assimilation.

Authors:  Germán Perdomo; Francisco J Navarro; Braulio Medina; Félix Machín; Paula Tejera; José M Siverio
Journal:  Plant Mol Biol       Date:  2002-10       Impact factor: 4.076

10.  Characterization of the Tuber borchii nitrate reductase gene and its role in ectomycorrhizae.

Authors:  M Guescini; R Pierleoni; F Palma; S Zeppa; L Vallorani; L Potenza; C Sacconi; G Giomaro; V Stocchi
Journal:  Mol Genet Genomics       Date:  2003-08-01       Impact factor: 3.291
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