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Literature DB >> 7788721

In vitro self-splicing reactions of chloroplast and mitochondrial group-I introns in Chlamydomonas eugametos and Chlamydomonas moewusii.

Abstract

The self-splicing activity of nine chloroplast group-I introns (CeLSU.1 to CeLSU.6, CepsbC.1, CepsbC.2 and CmpsaB.1) and of one mitochondrial group-I intron (CmmtLSU.1) from the interfertile green algae Chlamydomonas eugametos and C. moewusii was examined using RNA templates produced by in vitro transcription of cloned DNA sequences. All introns, with the exception of the mobile intron CeLSU.5 encoding the site-specific I-CeuI endonuclease, were found to catalyze their own splicing in the absence of proteins. The introns that proved to be the best substrates under the conditions employed are CeLSU.1, CeLSU.3, CeLSU.4, CepsbC.1 and CmmtLSU.1. The implications of our results for the origin and spread of group-I introns in the organellar genomes of green algae are discussed.

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Year: 1995 PMID： 7788721 DOI： 10.1007/bf00313432

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

34 in total

Review 1. Involvement of aminoacyl-tRNA synthetases and other proteins in group I and group II intron splicing.

Authors: A M Lambowitz; P S Perlman
Journal: Trends Biochem Sci Date: 1990-11 Impact factor: 13.807

2. In vitro self-splicing reactions of the chloroplast group I intron Cr.LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement.

Authors: A J Thompson; D L Herrin
Journal: Nucleic Acids Res Date: 1991-12-11 Impact factor: 16.971

3. Self-splicing of yeast mitochondrial ribosomal and messenger RNA precursors.

Authors: G van der Horst; H F Tabak
Journal: Cell Date: 1985-04 Impact factor: 41.582

Review 4. Conserved sequences and structures of group I introns: building an active site for RNA catalysis--a review.

Authors: T R Cech
Journal: Gene Date: 1988-12-20 Impact factor: 3.688

5. Mitotic recombination in germ cells generated two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbm6.

Authors: J Geliebter; R A Zeff; R W Melvold; S G Nathenson
Journal: Proc Natl Acad Sci U S A Date: 1986-05 Impact factor: 11.205

Review 6. Introns as mobile genetic elements.

Authors: A M Lambowitz; M Belfort
Journal: Annu Rev Biochem Date: 1993 Impact factor: 23.643

7. Autocatalytic activities of intron 5 of the cob gene of yeast mitochondria.

Authors: S Partono; A S Lewin
Journal: Mol Cell Biol Date: 1988-06 Impact factor: 4.272

8. Six group I introns and three internal transcribed spacers in the chloroplast large subunit ribosomal RNA gene of the green alga Chlamydomonas eugametos.

Authors: M Turmel; J Boulanger; M N Schnare; M W Gray; C Lemieux
Journal: J Mol Biol Date: 1991-03-20 Impact factor: 5.469

9. Two group I introns with long internal open reading frames in the chloroplast psbA gene of Chlamydomonas moewusii.

Authors: M Turmel; J Boulanger; C Lemieux
Journal: Nucleic Acids Res Date: 1989-05-25 Impact factor: 16.971

10. The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals.

Authors: J Hiratsuka; H Shimada; R Whittier; T Ishibashi; M Sakamoto; M Mori; C Kondo; Y Honji; C R Sun; B Y Meng
Journal: Mol Gen Genet Date: 1989-06

5 in total

5. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.

Authors: Decai Tuo; Wentao Shen; Pu Yan; Xiaoying Li; Peng Zhou
Journal: Viruses Date: 2015-12-01 Impact factor: 5.048