Literature DB >> 7785783

A quantitative test for copper using bicinchoninic acid.

A J Brenner1, E D Harris.   

Abstract

We describe a direct colorimetric assay for copper in serum and biological samples using 2,2'-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 x 10(3) liter mol-1 cm-1 and 4.6 x 10(4) liter mol-1 cm-1, respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7785783     DOI： 10.1006/abio.1995.1194

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  45 in total

1.  Acid regulates inflammatory response in a rat model of induction of gastric ulcer recurrence by interleukin 1beta.

Authors:  T Watanabe; K Higuchi; K Tominaga; Y Fujiwara; T Arakawa
Journal:  Gut       Date:  2001-06       Impact factor: 23.059

2.  Essential role of copper in the activity and regular periodicity of a recombinant, tumor-associated, cell surface, growth-related and time-keeping hydroquinone (NADH) oxidase with protein disulfide-thiol interchange activity (ENOX2).

Authors:  Xiaoyu Tang; P-J Chueh; Ziying Jiang; Sara Layman; Berdine Martin; Chinpal Kim; Dorothy M Morré; D James Morré
Journal:  J Bioenerg Biomembr       Date:  2010-10-05       Impact factor: 2.945

3.  Thermal stability effects of removing the type-2 copper ligand His306 at the interface of nitrite reductase subunits.

Authors:  Andrea Stirpe; Luigi Sportelli; Hein Wijma; Martin Ph Verbeet; Rita Guzzi
Journal:  Eur Biophys J       Date:  2007-03-16       Impact factor: 1.733

4.  Signal transduction by the global regulator RegB is mediated by a redox-active cysteine.

Authors:  Lee R Swem; Brian J Kraft; Danielle L Swem; Aaron T Setterdahl; Shinji Masuda; David B Knaff; Jeffrey M Zaleski; Carl E Bauer
Journal:  EMBO J       Date:  2003-09-15       Impact factor: 11.598

5.  Single-molecule study of metalloregulator CueR-DNA interactions using engineered Holliday junctions.

Authors:  Nesha May Andoy; Susanta K Sarkar; Qi Wang; Debashis Panda; Jaime J Benítez; Aleksandr Kalininskiy; Peng Chen
Journal:  Biophys J       Date:  2009-08-05       Impact factor: 4.033

6.  Comparison of the ligand-binding properties of native and copper-less cytochromes bo from Escherichia coli.

Authors:  A J Moody; R Mitchell; A E Jeal; P R Rich
Journal:  Biochem J       Date:  1997-06-15       Impact factor: 3.857

7.  The mechanism of Cu+ transport ATPases: interaction with CU+ chaperones and the role of transient metal-binding sites.

Authors:  Teresita Padilla-Benavides; Courtney J McCann; José M Argüello
Journal:  J Biol Chem       Date:  2012-11-26       Impact factor: 5.157

8.  Interactions of copper with glycated proteins: possible involvement in the etiology of diabetic neuropathy.

Authors:  John W Eaton; Mingwei Qian
Journal:  Mol Cell Biochem       Date:  2002 May-Jun       Impact factor: 3.396

9.  Copper Reduction and Contact Killing of Bacteria by Iron Surfaces.

Authors:  Salima Mathews; Ranjeet Kumar; Marc Solioz
Journal:  Appl Environ Microbiol       Date:  2015-07-06       Impact factor: 4.792

10.  Copper incorporation into recombinant CotA laccase from Bacillus subtilis: characterization of fully copper loaded enzymes.

Authors:  Paulo Durão; Zhenjia Chen; André T Fernandes; Peter Hildebrandt; Daniel H Murgida; Smilja Todorovic; Manuela M Pereira; Eduardo P Melo; Lígia O Martins
Journal:  J Biol Inorg Chem       Date:  2007-10-24       Impact factor: 3.358
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.