| Literature DB >> 7785768 |
A M Raizis1, F Schmitt, J P Jost.
Abstract
The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.Entities:
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Year: 1995 PMID： 7785768 DOI： 10.1006/abio.1995.1204
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365