| Literature DB >> 7780582 |
F Bonté1, P Pinguet, J M Chevalier, A Meybeck.
Abstract
An optimized gradient enabling the separation of all stratum corneum lipids by automated multiple development on HPTLC plates is presented. An initial isocratic step separates sebum lipids. This is followed by a 25-step development using a gradient with a polarity range of methanol-water to hexane. Application to in-vivo extracted and isolated stratum corneum lipids demonstrates the possible quantification of the lipid classes with a "one-experiment" separation.Entities:
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Year: 1995 PMID： 7780582 DOI： 10.1016/0378-4347(94)00480-s
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495