Literature DB >> 7779401

A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer.

G S Goldberg1, J F Bechberger, C C Naus.   

Abstract

We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

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Year:  1995        PMID： 7779401

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  52 in total

1.  ATP-mediated glia signaling.

Authors:  M L Cotrina; J H Lin; J C López-García; C C Naus; M Nedergaard
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2.  Dendritic fibroblasts in three-dimensional collagen matrices.

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Journal:  Mol Biol Cell       Date:  2003-02       Impact factor: 4.138

3.  Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein.

Authors:  B Teubner; J Degen; G Söhl; M Güldenagel; F F Bukauskas; E B Trexler; V K Verselis; C I De Zeeuw; C G Lee; C A Kozak; E Petrasch-Parwez; R Dermietzel; K Willecke
Journal:  J Membr Biol       Date:  2000-08-01       Impact factor: 1.843

4.  Adult-derived liver stem cells acquire a cardiomyocyte structural and functional phenotype ex vivo.

Authors:  Barbara J Muller-Borer; Wayne E Cascio; Page A W Anderson; John N Snowwaert; James R Frye; Niyati Desai; Gwyn L Esch; Joe A Brackham; C Robert Bagnell; William B Coleman; Joe W Grisham; Nadia N Malouf
Journal:  Am J Pathol       Date:  2004-07       Impact factor: 4.307

5.  Tetracycline-regulated expression enables purification and functional analysis of recombinant connexin channels from mammalian cells.

Authors:  Irina V Koreen; Wafaa A Elsayed; Yu J Liu; Andrew L Harris
Journal:  Biochem J       Date:  2004-10-01       Impact factor: 3.857

6.  A transient diffusion model yields unitary gap junctional permeabilities from images of cell-to-cell fluorescent dye transfer between Xenopus oocytes.

Authors:  Johannes M Nitsche; Hou-Chien Chang; Paul A Weber; Bruce J Nicholson
Journal:  Biophys J       Date:  2004-04       Impact factor: 4.033

7.  Selective esterase-ester pair for targeting small molecules with cellular specificity.

Authors:  Lin Tian; Yunlei Yang; Laura M Wysocki; Alma C Arnold; Amy Hu; Balaji Ravichandran; Scott M Sternson; Loren L Looger; Luke D Lavis
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-12       Impact factor: 11.205

8.  Investigation of connexin 43 uncoupling and prolongation of the cardiac QRS complex in preclinical and marketed drugs.

Authors:  M P Burnham; P M Sharpe; C Garner; R Hughes; C E Pollard; J Bowes
Journal:  Br J Pharmacol       Date:  2014-08-13       Impact factor: 8.739

9.  Connexin mediates gap junction-independent resistance to cellular injury.

Authors:  Jane H-C Lin; Jay Yang; Shujun Liu; Takahiro Takano; Xiaohai Wang; Qun Gao; Klaus Willecke; Maiken Nedergaard
Journal:  J Neurosci       Date:  2003-01-15       Impact factor: 6.167

10.  Gap junctional communication and pharmacological heterogeneity in astrocytes cultured from the rat striatum.

Authors:  L Venance; J Prémont; J Glowinski; C Giaume
Journal:  J Physiol       Date:  1998-07-15       Impact factor: 5.182
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