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Literature DB >> 7778787

A fluorescence assay for geranylgeranyl transferase type I.

W C Pickett¹, F L Zhang, C Silverstrim, S R Schow, M M Wick, S S Kerwar.

Abstract

A new fluorescence assay for measuring the activity of geranylgeranyl transferase (type I) is described. It does not require the use of either radiolabeled geranylgeranyl diphosphate or the purified recombinant Ras protein substrate with the carboxy terminal sequence of CVLL. Dansyl GCVLL and unlabeled geranylgeranyl diphosphate are used as substrates. The Km for Dansyl GCVLL and for geranylgeranyl diphosphate is 5 microM and 800 nM, respectively. At equimolar concentrations, enzymatic activity is higher when Dansyl GCVLL is used as a substrate compared to Dansyl GCVII. Dansyl GCVLS, a substrate for farnesyl transferase, is inactive in this assay. CVFL is a competitive inhibitor of geranylgeranyl transferase and exhibits a Ki of 200 nM.

Entities: Chemical

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Year: 1995 PMID： 7778787 DOI： 10.1006/abio.1995.1108

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

6 in total

1. Synthesis and evaluation of 3- and 7-substituted geranylgeranyl pyrophosphate analogs.

Authors: Michelle Maynor; Sarah A Scott; Emily L Rickert; Richard A Gibbs
Journal: Bioorg Med Chem Lett Date: 2008-02-12 Impact factor: 2.823

2. Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I.

Authors: F L Zhang; P J Casey
Journal: Biochem J Date: 1996-12-15 Impact factor: 3.857

A fluorescence assay for geranylgeranyl transferase type I.

1. Synthesis and evaluation of 3- and 7-substituted geranylgeranyl pyrophosphate analogs.

2. Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I.

3. TrkB-mediated activation of geranylgeranyltransferase I promotes dendritic morphogenesis.

4. Geranyl and neryl triazole bisphosphonates as inhibitors of geranylgeranyl diphosphate synthase.

5. Prenylation of Axonally Translated Rac1 Controls NGF-Dependent Axon Growth.

6. Efficient farnesylation of an extended C-terminal C(x)₃X sequence motif expands the scope of the prenylated proteome.

A fluorescence assay for geranylgeranyl transferase type I.

1. Synthesis and evaluation of 3- and 7-substituted geranylgeranyl pyrophosphate analogs.

2. Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I.

3. TrkB-mediated activation of geranylgeranyltransferase I promotes dendritic morphogenesis.

4. Geranyl and neryl triazole bisphosphonates as inhibitors of geranylgeranyl diphosphate synthase.

5. Prenylation of Axonally Translated Rac1 Controls NGF-Dependent Axon Growth.

6. Efficient farnesylation of an extended C-terminal C(x)3X sequence motif expands the scope of the prenylated proteome.

6. Efficient farnesylation of an extended C-terminal C(x)₃X sequence motif expands the scope of the prenylated proteome.