Literature DB >> 7778786

Simple and efficient method for isolation and measurement of cyanobacterial hepatotoxins by plant tests (Sinapis alba L.).

P Kós1, G Gorzó, G Surányi, G Borbély.   

Abstract

A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S. alba L. seedling test in the quantitative assessment of the toxin with an IC50 of 3 micrograms ml-1 instead of the mouse intraperitoneal test.

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Year:  1995        PMID： 7778786     DOI： 10.1006/abio.1995.1106

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  Microcystin-LR, a protein phosphatase inhibitor, induces alterations in mitotic chromatin and microtubule organization leading to the formation of micronuclei in Vicia faba.

Authors:  Dániel Beyer; Ildikó Tándor; Zoltán Kónya; Róbert Bátori; Janos Roszik; György Vereb; Ferenc Erdodi; Gábor Vasas; Márta M-Hamvas; Károly Jambrovics; Csaba Máthé
Journal:  Ann Bot       Date:  2012-07-20       Impact factor: 4.357

2.  Cyanobacterial extracts containing microcystins affect the growth, nodulation process and nitrogen uptake of faba bean (Vicia faba L., Fabaceae).

Authors:  Majida Lahrouni; Khalid Oufdou; Mustapha Faghire; Alvaro Peix; Fatima El Khalloufi; Vitor Vasconcelos; Brahim Oudra
Journal:  Ecotoxicology       Date:  2011-11-25       Impact factor: 2.823

3.  Novel fluorochromes label tonoplast in living plant cells and reveal changes in vacuolar organization after treatment with protein phosphatase inhibitors.

Authors:  Miklós Nagy; Sándor Kéki; Dávid Rácz; Jaideep Mathur; György Vereb; Tamás Garda; Márta M-Hamvas; François Chaumont; Károly Bóka; Béla Böddi; Csongor Freytag; Gábor Vasas; Csaba Máthé
Journal:  Protoplasma       Date:  2017-12-12       Impact factor: 3.356

4.  Physiological and biochemical defense reactions of Vicia faba L.-Rhizobium symbiosis face to chronic exposure to cyanobacterial bloom extract containing microcystins.

Authors:  Majida Lahrouni; Khalid Oufdou; Fatima El Khalloufi; Mohamed Baz; Alejandro Lafuente; Mohammed Dary; Eloisa Pajuelo; Brahim Oudra
Journal:  Environ Sci Pollut Res Int       Date:  2013-02-17       Impact factor: 4.223

5.  The solar photo-Fenton process at neutral pH applied to microcystin-LR degradation: Fe2+, H2O2 and reaction matrix effects.

Authors:  Joicy Micheletto; Mariana Almeida de Torres; Vinícius de Carvalho S de Paula; Vânia Eloiza Cerutti; Thomaz Aurélio Pagioro; Quezia Bezerra Cass; Lucia Regina R Martins; Marcus Vinicius de Liz; Adriane Martins de Freitas
Journal:  Photochem Photobiol Sci       Date:  2020-07-03       Impact factor: 3.982

6.  Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila).

Authors:  Jianzhong Chen; Jian Dai; Haiyang Zhang; Chenyi Wang; Guoqing Zhou; Zhiping Han; Zhili Liu
Journal:  Ecotoxicology       Date:  2010-01-06       Impact factor: 2.823

7.  CyanoHAB occurrence and water irrigation cyanotoxin contamination: ecological impacts and potential health risks.

Authors:  Sana Saqrane; Brahim Oudra
Journal:  Toxins (Basel)       Date:  2009-11-25       Impact factor: 4.546

8.  Exposure of Lycopersicon esculentum to microcystin-LR: effects in the leaf proteome and toxin translocation from water to leaves and fruits.

Authors:  Daniel Gutiérrez-Praena; Alexandre Campos; Joana Azevedo; Joana Neves; Marisa Freitas; Remédios Guzmán-Guillén; Ana María Cameán; Jenny Renaut; Vitor Vasconcelos
Journal:  Toxins (Basel)       Date:  2014-06-11       Impact factor: 4.546

Review 9.  Subcellular Alterations Induced by Cyanotoxins in Vascular Plants-A Review.

Authors:  Csaba Máthé; Márta M-Hamvas; Gábor Vasas; Tamás Garda; Csongor Freytag
Journal:  Plants (Basel)       Date:  2021-05-14

Review 10.  Microcystin-LR and cylindrospermopsin induced alterations in chromatin organization of plant cells.

Authors:  Csaba Máthé; Márta M-Hamvas; Gábor Vasas
Journal:  Mar Drugs       Date:  2013-09-30       Impact factor: 5.118
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