Literature DB >> 7776959

Production of native creatine kinase B in insect cells using a baculovirus expression vector.

Y J de Kok1, M P Geurds, E A Sistermans, M Usmany, J M Vlak, B Wieringa.   

Abstract

A full-length human creatine kinase B (B-CK) cDNA was used to produce a recombinant baculovirus (AcDZ1-BCK). Sf9 cells infected with this recombinant expressed a homodimeric protein composed of 43 kDa subunits which, under optimal conditions, formed up to 30% of the total soluble cellular protein. Upon analysis by PAGE, zymogram assay and gel filtration chromatography the recombinant protein behaved like authentic dimeric human BB-CK protein. Studies with a newly produced monoclonal antibody (CK-BYK/21E10) directed against an epitope in the N-terminus of the protein confirmed the identity of the product. The recombinant BB-CK protein was purified to over 99% homogeneity from the total protein extract of AcDZ1-CKB infected cells in one single step involving anion exchange column chromatography on MonoQ in FPLC. Dialysed protein had a specific activity of 239 U/mg protein.

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Year:  1995        PMID： 7776959     DOI： 10.1007/bf00925927

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  29 in total

1.  Regulation of creatine phosphokinase B activity by protein kinase C.

Authors:  K Chida; M Tsunenaga; K Kasahara; Y Kohno; T Kuroki
Journal:  Biochem Biophys Res Commun       Date:  1990-11-30       Impact factor: 3.575

2.  Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector.

Authors:  D Zuidema; A Schouten; M Usmany; A J Maule; G J Belsham; J Roosien; E C Klinge-Roode; J W van Lent; J M Vlak
Journal:  J Gen Virol       Date:  1990-10       Impact factor: 3.891

3.  Rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures.

Authors:  K Alvares; R J Widrow; G M Abu-Jawdeh; J V Schmidt; A V Yeldandi; M S Rao; J K Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Dideoxy sequencing method using denatured plasmid templates.

Authors:  M Hattori; Y Sakaki
Journal:  Anal Biochem       Date:  1986-02-01       Impact factor: 3.365

Review 6.  The creatine-creatine phosphate energy shuttle.

Authors:  S P Bessman; C L Carpenter
Journal:  Annu Rev Biochem       Date:  1985       Impact factor: 23.643

7.  Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction.

Authors:  V K Sarin; S B Kent; J P Tam; R B Merrifield
Journal:  Anal Biochem       Date:  1981-10       Impact factor: 3.365

8.  Isoenzymes of creatine phosphokinase in tissue extracts and in normal and pathological sera.

Authors:  K J van der Veen; A F Willebrands
Journal:  Clin Chim Acta       Date:  1966-03       Impact factor: 3.786

9.  The creatine kinase system in normal and diseased human myocardium.

Authors:  J S Ingwall; M F Kramer; M A Fifer; B H Lorell; R Shemin; W Grossman; P D Allen
Journal:  N Engl J Med       Date:  1985-10-24       Impact factor: 91.245

10.  Comparison of four bifunctional reagents for coupling peptides to proteins and the effect of the three moieties on the immunogenicity of the conjugates.

Authors:  J M Peeters; T G Hazendonk; E C Beuvery; G I Tesser
Journal:  J Immunol Methods       Date:  1989-06-02       Impact factor: 2.303
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  4 in total

1.  Co-localization and functional coupling of creatine kinase B and gastric H+/K(+)-ATPase on the apical membrane and the tubulovesicular system of parietal cells.

Authors:  E A Sistermans; C H Klaassen; W Peters; H G Swarts; P H Jap; J J De Pont; B Wieringa
Journal:  Biochem J       Date:  1995-10-15       Impact factor: 3.857

2.  Tissue- and cell-specific distribution of creatine kinase B: a new and highly specific monoclonal antibody for use in immunohistochemistry.

Authors:  E A Sistermans; Y J de Kok; W Peters; L A Ginsel; P H Jap; B Wieringa
Journal:  Cell Tissue Res       Date:  1995-05       Impact factor: 5.249

3.  Mice lacking brain-type creatine kinase activity show defective thermoregulation.

Authors:  Femke Streijger; Helma Pluk; Frank Oerlemans; Gaby Beckers; Antonio C Bianco; Miriam O Ribeiro; Bé Wieringa; Catharina E E M Van der Zee
Journal:  Physiol Behav       Date:  2009-02-10

4.  Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility.

Authors:  Jan W P Kuiper; Remco van Horssen; Frank Oerlemans; Wilma Peters; Michiel M T van Dommelen; Mariska M te Lindert; Timo L M ten Hagen; Edwin Janssen; Jack A M Fransen; Bé Wieringa
Journal:  PLoS One       Date:  2009-03-31       Impact factor: 3.240
  4 in total

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