Ubenimex activates the E-cadherin-mediated adhesion of a breast cancer cell line YMB-S.

It has been reported that ubenimex, a biological response modifier, has a direct anti-tumor effect. To clarify the mechanism involved, we examined the effects of ubenimex on the growth and adhesive property of a breast cancer cell line YMB-S. The cells proliferate in a floating manner without aggregation in normal complete medium. Ubenimex induced cell-cell and cell-surface adhesion of the cells accompanied with growth suppression. E-Cadherin localized at cell-cell contact sites of adhered cells, and anti-E-cadherin antibody inhibited the adhesion. Both Western blot analysis and binding assay disclosed that there was no apparent difference between E-cadherin levels of the cells before and after the treatment with ubenimex. These results indicate that ubenimex inhibits the proliferation of YMB-S cells and augments cell-to-cell adhesion through the induction of E-cadherin-mediated adhesion resulting from the functional activation of pre-expressed but inefficient E-cadherin.