| Literature DB >> 7772044 |
Abstract
Chicken egg yolk biotin-binding protein-I (BBP-I) has been purified to homogeneity along with the tetrameric BBP-II by a common protocol. The purification includes delipidation of egg yolk by butanol extraction, DEAE-Sephacel chromatography, treatment with guanidinium chloride and biotin-aminohexyl-Sepharose affinity chromatography. The identity of purified BBP-I was ascertained by its physicochemical properties as well as by its immunological cross-reactivity and precursor-product relationship with BBP-II.Entities:
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Year: 1995 PMID: 7772044 PMCID: PMC1136964 DOI: 10.1042/bj3080573
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857