| Literature DB >> 7770631 |
Y Murakami1, K Koshimura, K Yamauchi, M Nishiki, J Tanaka, H Furuya, T Miyake, Y Kato.
Abstract
Effect of pituitary adenylate cyclase activating polypeptide (PACAP) on growth hormone (GH) release from GH3 cells was studied in a dynamic superfusion system. PACAP-38 and PACAP-27 stimulated GH release from superfused GH3 cells. The stimulatory effect of PACAP-38 was comparable to those of vasoactive intestinal polypeptide (VIP) and PACAP-27 at a concentration of 1 nM, but the duration of action was more prolonged in PACAP-38 than in the other two peptides. PACAP(6-38), a selective antagonist of PACAP, as well as a VIP antagonist blunted the GH release induced by PACAP-38 and VIP. An antagonist of GH-releasing factor (GRF) at a concentration of 1 microM, however, did not affect the GH release induced by PACAP-38. These findings suggest that PACAP and VIP stimulate GH release from GH3 cells through type II PACAP receptor but not through the GRF receptors.Entities:
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Year: 1995 PMID： 7770631 DOI： 10.1016/0167-0115(95)00003-t
Source DB: PubMed Journal: Regul Pept ISSN： 0167-0115