Literature DB >> 7769027

Detection of Epstein-Barr virus-specific antibodies by means of baculovirus-expressed EBV gp125.

D Sánchez-Martínez1, J L Patton, J A Stewart, P E Pellett.   

Abstract

A major antigenic component of the Epstein-Barr virus viral capsid antigen (VCA) complex is the glycoprotein, gp125. Baculovirus-expressed gp125 reacted with Epstein-Barr virus IgG antibodies in a panel of 44 serum specimens using an immunoblot assay with over 97% sensitivity, and 100% specificity as compared to anti-VCA reactivity in an immunofluorescence assay. In addition, no evidence for cross-reactivity was seen in reactions with members of a panel of human serum specimens of known reactivity with each of the other known human herpesviruses. Thus, baculovirus-expressed gp125 should prove a stable platform on which new Epstein-Barr virus-specific serodiagnostic tests can be built.

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Year:  1995        PMID： 7769027     DOI： 10.1016/0166-0934(94)00157-c

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

1.  Glycoprotein gp110 of Epstein-Barr virus determines viral tropism and efficiency of infection.

Authors:  B Neuhierl; R Feederle; W Hammerschmidt; H J Delecluse
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-30       Impact factor: 11.205

2.  Antibodies to lytic infection proteins in lymphocryptovirus-infected rhesus macaques: a model for humoral immune responses to epstein-barr virus infection.

Authors:  Nina Orlova; Mark H Fogg; Angela Carville; Fred Wang
Journal:  Clin Vaccine Immunol       Date:  2011-07-06

3.  Comparison of serologic assays for detection of antibodies against human herpesvirus 8.

Authors:  J L Corchero; E C Mar; T J Spira; P E Pellett; N Inoue
Journal:  Clin Diagn Lab Immunol       Date:  2001-09

4.  Clinical reliability of IgG, IgA, and IgM antibodies in detecting Epstein-Barr virus at different stages of infection with a commercial nonrecombinant polyantigenic ELISA.

Authors:  J Gutiérrez; M J Vergara; G Piédrola; M C Maroto
Journal:  J Clin Lab Anal       Date:  1999       Impact factor: 2.352

5.  Serodiagnosis of Epstein-Barr virus infection by using recombinant viral capsid antigen fragments and autologous gene fusion.

Authors:  W Hinderer; D Lang; M Rothe; R Vornhagen; H H Sonneborn; H Wolf
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

6.  Immunofluorescence assay for detection of human metapneumovirus-specific antibodies by use of baculovirus-expressed fusion protein.

Authors:  Nobuhisa Ishiguro; Takashi Ebihara; Rika Endo; Xiaoming Ma; Ryo Shirotsuki; Susumu Ochiai; Hiroaki Ishiko; Hideaki Kikuta
Journal:  Clin Diagn Lab Immunol       Date:  2005-01

7.  Epstein-Barr virus glycoprotein gH/gL antibodies complement IgA-viral capsid antigen for diagnosis of nasopharyngeal carcinoma.

Authors:  Rui-Chen Li; Yong Du; Qiu-Yao Zeng; Lin-Quan Tang; Hua Zhang; Yan Li; Wan-Li Liu; Qian Zhong; Mu-Sheng Zeng; Xiao-Ming Huang
Journal:  Oncotarget       Date:  2016-03-29
  7 in total

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