Literature DB >> 7766194

Purification and partial characterization of a spore cortex-lytic enzyme of Clostridium perfringens S40 spores.

S Miyata1, R Moriyama, K Sugimoto, S Makino.   

Abstract

A spore cortex-lytic enzyme was purified in an active form from the exudate of fully germinated spores of Clostridium perfringens S40. The enzyme caused attenuation of absorbance in coatless spore suspensions and phase-darkening of the spores, but had minimal activity on isolated peptidoglycan fragments. The enzyme was identified as a 31 kDa protein which is probably an N-acetylmuramyl-L-alanine amidase. The amino-terminal 15 residues of the enzyme were: VLPEPVVPEYIVVHN.

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Year:  1995        PMID： 7766194     DOI： 10.1271/bbb.59.514

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  13 in total

1.  Localization of a germinant receptor protein (GerBA) to the inner membrane of Bacillus subtilis spores.

Authors:  M Paidhungat; P Setlow
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

2.  A germination-specific spore cortex-lytic enzyme from Bacillus cereus spores: cloning and sequencing of the gene and molecular characterization of the enzyme.

Authors:  R Moriyama; S Kudoh; S Miyata; S Nonobe; A Hattori; S Makino
Journal:  J Bacteriol       Date:  1996-09       Impact factor: 3.490

Review 3.  Sporulation and Germination in Clostridial Pathogens.

Authors:  Aimee Shen; Adrianne N Edwards; Mahfuzur R Sarker; Daniel Paredes-Sabja
Journal:  Microbiol Spectr       Date:  2019-11

4.  Partial characterization of an enzyme fraction with protease activity which converts the spore peptidoglycan hydrolase (SleC) precursor to an active enzyme during germination of Clostridium perfringens S40 spores and analysis of a gene cluster involved in the activity.

Authors:  S Shimamoto; R Moriyama; K Sugimoto; S Miyata; S Makino
Journal:  J Bacteriol       Date:  2001-06       Impact factor: 3.490

5.  Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.

Authors:  R Moriyama; H Fukuoka; S Miyata; S Kudoh; A Hattori; S Kozuka; Y Yasuda; K Tochikubo; S Makino
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

6.  Molecular characterization of a germination-specific muramidase from Clostridium perfringens S40 spores and nucleotide sequence of the corresponding gene.

Authors:  Y Chen; S Miyata; S Makino; R Moriyama
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

7.  A novel spore peptidoglycan hydrolase of Bacillus cereus: biochemical characterization and nucleotide sequence of the corresponding gene, sleL.

Authors:  Y Chen; S Fukuoka; S Makino
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

8.  Peptidoglycan structural dynamics during germination of Bacillus subtilis 168 endospores.

Authors:  A Atrih; P Zöllner; G Allmaier; M P Williamson; S J Foster
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

9.  A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to L-alanine-mediated germination.

Authors:  R Moriyama; A Hattori; S Miyata; S Kudoh; S Makino
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

10.  Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium.

Authors:  Glenn E Tillman; Mustafa Simmons; Johnna K Garrish; Bruce S Seal
Journal:  Arch Microbiol       Date:  2013-08-11       Impact factor: 2.552
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