Literature DB >> 776610

Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.

U Nudel, H Soreq, U Z Littauer.   

Abstract

Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)-cellulose chromatography at 22 degrees C and at 4 degrees C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as poly(A)-free globin MRNA.

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Year:  1976        PMID： 776610     DOI： 10.1111/j.1432-1033.1976.tb10279.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  34 in total

1.  Yeast transcripts cleaved by an internal ribozyme provide new insight into the role of the cap and poly(A) tail in translation and mRNA decay.

Authors:  Stacie Meaux; Ambro Van Hoof
Journal:  RNA       Date:  2006-05-19       Impact factor: 4.942

2.  Translation and a 42-nucleotide segment within the coding region of the mRNA encoded by the MAT alpha 1 gene are involved in promoting rapid mRNA decay in yeast.

Authors:  R Parker; A Jacobson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

3.  Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translation.

Authors:  D R Gallie; W J Lucas; V Walbot
Journal:  Plant Cell       Date:  1989-03       Impact factor: 11.277

4.  Increase in the levels of activity of polyadenylic acid-metabolizing enzymes following phytohaemagglutinin stimulation of human lymphocytes.

Authors:  N C Courtis; T T Trangas; C M Tsiapalis
Journal:  Mol Cell Biochem       Date:  1987-05       Impact factor: 3.396

5.  Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.

Authors:  H Soreq; R Parvari; I Silman
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

6.  The ribonucleases of bovine skeletal muscle.

Authors:  G E Davies; T P Karpetsky; C C Levy
Journal:  Biochem J       Date:  1980-08-01       Impact factor: 3.857

7.  Messenger RNA stability in Saccharomyces cerevisiae: the influence of translation and poly(A) tail length.

Authors:  T C Santiago; A J Bettany; I J Purvis; A J Brown
Journal:  Nucleic Acids Res       Date:  1987-03-25       Impact factor: 16.971

8.  Post-transcriptional modification of the poly(A) length of galactose-1-phosphate uridyl transferase mRNA in Saccharomyces cerevisiae.

Authors:  C A Saunders; K A Bostian; H O Halvorson
Journal:  Nucleic Acids Res       Date:  1980-09-11       Impact factor: 16.971

9.  Encephalomyocarditis virus RNA. II. Polyadenylic acid requirement for efficient translation.

Authors:  D E Hruby; W K Roberts
Journal:  J Virol       Date:  1977-08       Impact factor: 5.103

10.  Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA isolation procedure.

Authors:  Y Gopalakrishna; D Langley; N Sarkar
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971
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